BMRB Entry 7114

Title:
1H, 13C, and 15N chemical shift assignment of Escherichia coli Maltose Binding Protein complexed with Beta-Cyclodextrin
Deposition date:
2006-05-17
Original release date:
2006-11-01
Authors:
Xu, Yingqi; Lin, Zhi; Zheng, Yu; Yang, Daiwen
Citation:

Citation: Xu, Yingqi; Zheng, Yu; Fan, JS; Yang, Daiwen. "A new strategy for structure determination of large proteins in solution without deuteration"  Nature Methods 3, 931-937 (2006).
PubMed: 17060917

Assembly members:

Assembly members:
maltose binding protein, polymer, 370 residues, Formula weight is not available
BCD, SACCHARIDE, 1134.984 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts1605
15N chemical shifts373
1H chemical shifts2411

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MBP1
2beta-cyclodextrin2

Entities:

Entity 1, MBP 370 residues - Formula weight is not available

1   LYSTHRGLUGLUGLYLYSLEUVALILETRP
2   ILEASNGLYASPLYSGLYTYRASNGLYLEU
3   ALAGLUVALGLYLYSLYSPHEGLULYSASP
4   THRGLYILELYSVALTHRVALGLUHISPRO
5   ASPLYSLEUGLUGLULYSPHEPROGLNVAL
6   ALAALATHRGLYASPGLYPROASPILEILE
7   PHETRPALAHISASPARGPHEGLYGLYTYR
8   ALAGLNSERGLYLEULEUALAGLUILETHR
9   PROASPLYSALAPHEGLNASPLYSLEUTYR
10   PROPHETHRTRPASPALAVALARGTYRASN
11   GLYLYSLEUILEALATYRPROILEALAVAL
12   GLUALALEUSERLEUILETYRASNLYSASP
13   LEULEUPROASNPROPROLYSTHRTRPGLU
14   GLUILEPROALALEUASPLYSGLULEULYS
15   ALALYSGLYLYSSERALALEUMETPHEASN
16   LEUGLNGLUPROTYRPHETHRTRPPROLEU
17   ILEALAALAASPGLYGLYTYRALAPHELYS
18   TYRGLUASNGLYLYSTYRASPILELYSASP
19   VALGLYVALASPASNALAGLYALALYSALA
20   GLYLEUTHRPHELEUVALASPLEUILELYS
21   ASNLYSHISMETASNALAASPTHRASPTYR
22   SERILEALAGLUALAALAPHEASNLYSGLY
23   GLUTHRALAMETTHRILEASNGLYPROTRP
24   ALATRPSERASNILEASPTHRSERLYSVAL
25   ASNTYRGLYVALTHRVALLEUPROTHRPHE
26   LYSGLYGLNPROSERLYSPROPHEVALGLY
27   VALLEUSERALAGLYILEASNALAALASER
28   PROASNLYSGLULEUALALYSGLUPHELEU
29   GLUASNTYRLEULEUTHRASPGLUGLYLEU
30   GLUALAVALASNLYSASPLYSPROLEUGLY
31   ALAVALALALEULYSSERTYRGLUGLUGLU
32   LEUALALYSASPPROARGILEALAALATHR
33   METGLUASNALAGLNLYSGLYGLUILEMET
34   PROASNILEPROGLNMETSERALAPHETRP
35   TYRALAVALARGTHRALAVALILEASNALA
36   ALASERGLYARGGLNTHRVALASPGLUALA
37   LEULYSASPALAGLNTHRARGILETHRLYS

Entity 2, beta-cyclodextrin - 1134.984 Da.

1   BCD

Samples:

sample_1: maltose binding protein 1.2 mM; beta-cyclodextrin

conditions_1: pH*: 7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
4D-15N,13C-edited NOESYsample_1not availableconditions_1
3D-CCH-TOCSYsample_1not availableconditions_1
TROSY-HNCAsample_1not availableconditions_1
4D-13C,13C-edited NOESYsample_1not availableconditions_1

Software:

SPARKY, UCSF - peak assignment

NMR spectrometers:

  • Bruker . 800 MHz

Related Database Links:

BMRB 25237 4354 4986 4987 6807
PDB
DBJ BAB38440 BAE78036 BAG79849 BAI28296 BAI33473
EMBL CAP78494 CAQ34383 CAR01012 CAR05669 CAR10711
GB AAB59056 AAB86559 AAB87675 AAC43128 AAC77004
REF NP_290668 NP_313044 NP_418458 NP_709885 NP_756856
SP P0AEX9 P0AEY0
AlphaFold P0AEY0 P0AEX9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks