BMRB Entry 7215

Title:
Backbone 1H Chemical Shift Assignments for J7
Deposition date:
2006-07-03
Original release date:
2007-01-09
Authors:
Sabo, Jennifer; Keizer, David; Feng, Zhi-Ping; Casey, Joanne; Parisi, Kathy; Coley, Andrew; Foley, Michael; Norton, Raymond
Citation:

Citation: Sabo, Jennifer; Keizer, David; Feng, Zhi-Ping; Casey, Joanne; Parisi, Kathy; Coley, Andrew; Foley, Michael; Norton, Raymond. "Mimotopes of Apical Membrane Antigen 1: Structures of Phage-Derived Peptides Recognized by the Inhibitory Monoclonal Antibody 4G2dc1 and Design of a More Active Analogue"  Infect. Immun. 75, 61-73 (2007).
PubMed: 17060469

Assembly members:

Assembly members:
J7, polymer, 21 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Enterobacteria phage M13   Taxonomy ID: 10870   Superkingdom: Viruses   Kingdom: not available   Genus/species: Inovirus Inovirus Enterobacteria phage

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
J7: LASLRKAFADTVPVRPPSNY G

Data sets:
Data typeCount
1H chemical shifts125
coupling constants16

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1J71

Entities:

Entity 1, J7 21 residues - Formula weight is not available

1   LEUALASERLEUARGLYSALAPHEALAASP
2   THRVALPROVALARGPROPROSERASNTYR
3   GLY

Samples:

sample_1: J7 1 mM

conditions_1: pH: 4.5; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1not availableconditions_1
2D 1H-1H DQF-COSYsample_1not availableconditions_1
2D 1H-1H NOESYsample_1not availableconditions_1

Software:

xwinnmr v3.5, Bruker Biospin -

XEASY v1.3.13 -

CYANA v1.0.6 -

X-PLOR NIH v2.9.3 -

NMR spectrometers:

  • Bruker Biospin AMX 500 MHz
  • Bruker Biospin DRX 600 MHz