BMRB Entry 7281

Title:
NMR STRUCTURE OF PROTEIN YJBR FROM ESCHERICHIA COLI;NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER226
Deposition date:
2006-08-29
Original release date:
2006-10-23
Authors:
SINGARAPU, KIRAN; GAOHUA, LIU; XIAO, RONG; SHETTY, KARISHMA; GAETANO, MONTELIONE; SZYPERSKI, THOMAS
Citation:

Citation: Singarapu, Kiran; Liu, Gaohua; Xiao, Rong; Bertonati, Claudia; Honig, Barry; Montelione, Gaetano; Szyperski, Thomas. "NMR structure of protein yjbR from Escherichia coli reveals 'double-wing' DNA binding motif."  Proteins 67, 501-504 (2007).
PubMed: 17266124

Assembly members:

Assembly members:
protein yjbR, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Data sets:
Data typeCount
13C chemical shifts507
15N chemical shifts113
1H chemical shifts845

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein yjbR monomer1

Entities:

Entity 1, protein yjbR monomer 126 residues - Formula weight is not available

1   METTHRILESERGLULEULEUGLNTYRCYS
2   METALALYSPROGLYALAGLUGLNSERVAL
3   HISASNASPTRPLYSALATHRGLNILELYS
4   VALGLUASPVALLEUPHEALAMETVALLYS
5   GLUVALGLUASNARGPROALAVALSERLEU
6   LYSTHRSERPROGLULEUALAGLULEULEU
7   ARGGLNGLNHISSERASPVALARGPROSER
8   ARGHISLEUASNLYSALAHISTRPSERTHR
9   VALTYRLEUASPGLYSERLEUPROASPSER
10   GLNILETYRTYRLEUVALASPALASERTYR
11   GLNGLNALAVALASNLEULEUPROGLUGLU
12   LYSARGLYSLEULEUVALGLNLEULEUGLU
13   HISHISHISHISHISHIS

Samples:

sample_1: protein yjbR, [U-13C; U-15N], 1.16 mM; DTT 100 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; NaN3 0.02%; D2O 5%; H2O 95%

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
4,3D, GFT HNNCABCAsample_1not availableconditions_1
4,3D, GFT CABCACONNHsample_1not availableconditions_1
4,3D, GFT HABCABCONNHsample_1not availableconditions_1
15N-13C, 3D NOESYsample_1not availableconditions_1
2D 1H13C HSQCsample_1not availableconditions_1
4,3D, GFT HCCH COSYsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D 1H, 13C aro NOESYsample_1not availableconditions_1
3D, HCCH COSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • VARIAN UNITY 750 MHz

Related Database Links:

PDB
DBJ BAB38462 BAE78059 BAG79875 BAI28319 BAI33496
EMBL CAP78523 CAQ34406 CAQ91581 CAR01037 CAR05706
GB AAC43151 AAC77027 AAG59255 AAN45569 AAN83473
REF NP_290690 NP_313066 NP_418481 NP_709862 NP_756899
SP P0AF50 P0AF51
AlphaFold P0AF50 P0AF51

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks