BMRB Entry 10047

Title:
Solution Structure of the Homeobox Domain of Human Homeodomain Leucine Zipper-Encoding Gene (Homez)
Deposition date:
2006-11-09
Original release date:
2008-08-13
Authors:
Kamatari, Y.; Tochio, N.; Nameki, N.; Miyamoto, K.; Li, H.; Kobayashi, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Kamatari, Y.; Tochio, N.; Nameki, N.; Miyamoto, K.; Li, H.; Kobayashi, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Homeobox Domain of Human Homeodomain Leucine Zipper-Encoding Gene (Homez)"  .

Assembly members:

Assembly members:
homeobox domain, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040126-87

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts330
15N chemical shifts73
1H chemical shifts513

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Homeobox leucine zipper protein Homez1

Entities:

Entity 1, Homeobox leucine zipper protein Homez 76 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSARGLYS
2   THRLYSGLUGLNLEUALAILELEULYSSER
3   PHEPHELEUGLNCYSGLNTRPALAARGARG
4   GLUASPTYRGLNLYSLEUGLUGLNILETHR
5   GLYLEUPROARGPROGLUILEILEGLNTRP
6   PHEGLYASPTHRARGTYRALALEULYSHIS
7   GLYGLNLEULYSTRPPHEARGASPASNALA
8   SERGLYPROSERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.8998, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA92681 BAC98171 BAG10069 BAG59479 BAG63292
GB AAH84701 AAH85694 AAH94669 AAI30393 AAM94347
REF NP_001171176 NP_065885 NP_690062 NP_898997 XP_001102279
SP Q80W88 Q8IX15 Q8K3E9
TPG DAA25845
AlphaFold Q80W88 Q8IX15 Q8K3E9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks