BMRB Entry 10072

Title:
Solution structure of RRM domain in HNRPC protein
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.
Citation:

Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of RRM domain in HNRPC protein"  .

Assembly members:

Assembly members:
RRM domain, polymer, 98 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040301-06

Data sets:
Data typeCount
13C chemical shifts376
15N chemical shifts89
1H chemical shifts605

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Heterogeneous nuclear ribonucleoproteins C1/C21

Entities:

Entity 1, Heterogeneous nuclear ribonucleoproteins C1/C2 98 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSTHRASP
2   PROARGSERMETASNSERARGVALPHEILE
3   GLYASNLEUASNTHRLEUVALVALLYSLYS
4   SERASPVALGLUALAILEPHESERLYSTYR
5   GLYLYSILEVALGLYCYSSERVALHISLYS
6   GLYPHEALAPHEVALGLNTYRVALASNGLU
7   ARGASNALAARGALAALAVALALAGLYGLU
8   ASPGLYARGMETILEALAGLYGLNVALLEU
9   ASPILEASNLEUALAALAGLUPROLYSVAL
10   ASNARGSERGLYPROSERSERGLY

Samples:

sample_1: RRM domain, [U-13C; U-15N], 0.8 mM; d-Tris-HCl(pH 7.0) 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v2.0.29, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB AAV66399 EDL42257 EDL42258
REF XP_005372243 XP_009989486 XP_012325381 XP_013226683

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks