BMRB Entry 10098

Title:
Solution Structure of The C1 Domain of The Human Diacylglycerol Kinase Delta
Deposition date:
2007-02-13
Original release date:
2008-08-15
Authors:
Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of The C1 Domain of The Human Diacylglycerol Kinase Delta"  .

Assembly members:

Assembly members:
C1 Domain, polymer, 84 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P030128-49

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts75
1H chemical shifts506

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Diacylglycerol kinase, delta1
2ZINC (II) ION, 12
3ZINC (II) ION, 22

Entities:

Entity 1, Diacylglycerol kinase, delta 84 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRTHRLEU
2   ALASERILEGLYLYSASPILEILEGLUASP
3   ALAASPGLYILEALAMETPROHISGLNTRP
4   LEUGLUGLYASNLEUPROVALSERALALYS
5   CYSTHRVALCYSASPLYSTHRCYSGLYSER
6   VALLEUARGLEUGLNASPTRPARGCYSLEU
7   TRPCYSLYSALAMETVALHISTHRSERCYS
8   LYSGLUSERLEULEUTHRLYSCYSSERGLY
9   PROSERSERGLY

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C1 Domain, [U-13C; U-15N], 1.05 mM; d-Tris-HCl 20 mM; NaCl 100 mM; NaN3 0.02%; ZnCl2 100 uM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA09766 BAA11134 BAC11809 BAE02082
GB AAY24321 EAW71047 EAW71048 EAW71049 EAW71050
REF NP_003639 NP_690618 XP_001114920 XP_001916296 XP_002918009
SP Q16760
AlphaFold Q16760

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks