BMRB Entry 10271

Title:
Solution structures of the fn3 domain of human collagen alpha-1(XX) chain
Deposition date:
2008-12-15
Original release date:
2009-12-14
Authors:
Sato, M.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the fn3 domain of human collagen alpha-1(XX) chain"  .

Assembly members:

Assembly members:
Fibronectin type III domain, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P051216-01

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts403
15N chemical shifts89
1H chemical shifts614

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fibronectin type III domain1

Entities:

Entity 1, Fibronectin type III domain 108 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEUALAPRO
2   PROARGHISLEUGLYPHESERASPVALSER
3   HISASPALAALAARGVALPHETRPGLUGLY
4   ALAPROARGPROVALARGLEUVALARGVAL
5   THRTYRVALSERSERGLUGLYGLYHISSER
6   GLYGLNTHRGLUALAPROGLYASNALATHR
7   SERALAMETLEUGLYPROLEUSERSERSER
8   THRTHRTYRTHRVALARGVALTHRCYSLEU
9   TYRPROGLYGLYGLYSERSERTHRLEUTHR
10   GLYARGVALTHRTHRLYSLYSALAPROSER
11   PROSERSERGLYPROSERSERGLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v2.0.17, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks