BMRB Entry 11045

Title:
Mhr1p-bound ssDNA
Deposition date:
2008-05-15
Original release date:
2009-10-15
Authors:
Shibata, Takehiko; Ito, Yutaka
Citation:

Citation: Masuda, Tokiha; Ito, Yutaka; Terada, Tohru; Shibata, Takehiko; Mikawa, Tsutomu. "A non-canonical DNA structure enables homologous recombination in various genetic systems."  J. Biol. Chem. 284, 30230-30239 (2009).
PubMed: 19729448

Assembly members:

Assembly members:
DNA (5'-D(*DTP*DAP*DCP*DG)-3'), polymer, 4 residues, 1231.30 Da.
Mhr1p, polymer, 226 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: obtained from a vendor

Data sets:
Data typeCount
1H chemical shifts37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (5'-D(*DTP*DAP*DCP*DG)-3') 11
2DNA (5'-D(*DTP*DAP*DCP*DG)-3') 21
3DNA (5'-D(*DTP*DAP*DCP*DG)-3') 31
4DNA (5'-D(*DTP*DAP*DCP*DG)-3') 41
5DNA (5'-D(*DTP*DAP*DCP*DG)-3') 51
6DNA (5'-D(*DTP*DAP*DCP*DG)-3') 61
7DNA (5'-D(*DTP*DAP*DCP*DG)-3') 71
8DNA (5'-D(*DTP*DAP*DCP*DG)-3') 81
9DNA (5'-D(*DTP*DAP*DCP*DG)-3') 91
10DNA (5'-D(*DTP*DAP*DCP*DG)-3') 101
11Mhr1p2

Entities:

Entity 1, DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 4 residues - 1231.30 Da.

1   DTDADCDG

Entity 2, Mhr1p 226 residues - Formula weight is not available

1   METLYSVALASNHISSERILESERARGPHE
2   ARGPROALASERTRPPHEGLULYSTHRLYS
3   ILEILEPROPROGLNVALTYRILEPHEARG
4   ASNLEUGLUTYRGLYGLNVALLEUTYRSER
5   GLNPHEPROASNPHESERGLNTHRGLNVAL
6   ASPLYSLEUPHEVALARGPROASNTRPSER
7   ASNARGLYSPROSERLEUARGARGASPILE
8   TRPLYSCYSMETCYSVALVALASNLEUGLN
9   ASNTYRLYSGLNSERVALHISLEUTYRGLN
10   ASNLEUCYSARGLEUARGTYRLEUARGASP
11   VALALAGLNARGLYSGLUSERASPLYSLEU
12   ARGLYSLYSASPSERASNGLYHISVALTRP
13   TYRSERGLYGLNTYRARGPROTHRTYRCYS
14   GLNGLUALAVALALAASPLEUARGGLUSER
15   LEULEULYSVALPHEGLUASNALATHRPRO
16   ALAGLULYSGLNTHRVALPROALALYSLYS
17   PROSERILETYRTRPGLUASPPROTRPARG
18   METGLYASPLYSASPLYSHISTRPASNTYR
19   ASPVALPHEASNALALEUGLYLEUGLUHIS
20   LYSLEUILEGLNARGVALGLYASNILEALA
21   ARGGLUGLUSERVALILELEULYSGLULEU
22   ALALYSLEUGLUSERHISPROTHRGLUGLN
23   THRGLUVALSERSERGLN

Samples:

sample_1: DNA (5'-D(*DTP*DAP*DCP*DG)-3') 0.5 mM; Mhr1p 0.05 mM; MgCl2 6.7 mM; NaCl 50 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 7.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H transferred NOESYsample_1isotropicsample_conditions_1
2D 1H-1H DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H ROESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - processing

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA88081 GAA22516
EMBL CAY78797
GB AAB64732 AAS56061 AHY75269 AJP37996 AJU58119
REF NP_010582
SP Q06630
TPG DAA12135
AlphaFold Q06630