BMRB Entry 11048

Title:
RecT-bound ssDNA
Deposition date:
2008-05-15
Original release date:
2009-10-15
Authors:
Shibata, Takehiko; Ito, Yutaka
Citation:

Citation: Masuda, Tokiha; Ito, Yutaka; Terada, Tohru; Shibata, Takehiko; Mikawa, Tsutomu. "A non-canonical DNA structure enables homologous recombination in various genetic systems."  J. Biol. Chem. 284, 30230-30239 (2009).
PubMed: 19729448

Assembly members:

Assembly members:
DNA (5'-D(*DTP*DAP*DCP*DG)-3'), polymer, 4 residues, 1231.30 Da.
ecRecT, polymer, 269 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: obtained from a vendor

Data sets:
Data typeCount
1H chemical shifts37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (5'-D(*DTP*DAP*DCP*DG)-3') 11
2DNA (5'-D(*DTP*DAP*DCP*DG)-3') 21
3DNA (5'-D(*DTP*DAP*DCP*DG)-3') 31
4DNA (5'-D(*DTP*DAP*DCP*DG)-3') 41
5DNA (5'-D(*DTP*DAP*DCP*DG)-3') 51
6DNA (5'-D(*DTP*DAP*DCP*DG)-3') 61
7DNA (5'-D(*DTP*DAP*DCP*DG)-3') 71
8DNA (5'-D(*DTP*DAP*DCP*DG)-3') 81
9DNA (5'-D(*DTP*DAP*DCP*DG)-3') 91
10DNA (5'-D(*DTP*DAP*DCP*DG)-3') 101
11ecRecT2

Entities:

Entity 1, DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 4 residues - 1231.30 Da.

1   DTDADCDG

Entity 2, ecRecT 269 residues - Formula weight is not available

1   METTHRLYSGLNPROPROILEALALYSALA
2   ASPLEUGLNLYSTHRGLNGLYASNARGALA
3   PROALAALAVALLYSASNSERASPVALILE
4   SERPHEILEASNGLNPROSERMETLYSGLU
5   GLNLEUALAALAALALEUPROARGHISMET
6   THRALAGLUARGMETILEARGILEALATHR
7   THRGLUILEARGLYSVALPROALALEUGLY
8   ASNCYSASPTHRMETSERPHEVALSERALA
9   ILEVALGLNCYSSERGLNLEUGLYLEUGLU
10   PROGLYSERALALEUGLYHISALATYRLEU
11   LEUPROPHEGLYASNLYSASNGLULYSSER
12   GLYLYSLYSASNVALGLNLEUILEILEGLY
13   TYRARGGLYMETILEASPLEUALAARGARG
14   SERGLYGLNILEALASERLEUSERALAARG
15   VALVALARGGLUGLYASPGLUPHESERPHE
16   GLUPHEGLYLEUASPGLULYSLEUILEHIS
17   ARGPROGLYGLUASNGLUASPALAPROVAL
18   THRHISVALTYRALAVALALAARGLEULYS
19   ASPGLYGLYTHRGLNPHEGLUVALMETTHR
20   ARGLYSGLNILEGLULEUVALARGSERLEU
21   SERLYSALAGLYASNASNGLYPROTRPVAL
22   THRHISTRPGLUGLUMETALALYSLYSTHR
23   ALAILEARGARGLEUPHELYSTYRLEUPRO
24   VALSERILEGLUILEGLNARGALAVALSER
25   METASPGLULYSGLUPROLEUTHRILEASP
26   PROALAASPSERSERVALLEUTHRGLYGLU
27   TYRSERVALILEASPASNSERGLUGLU

Samples:

sample_1: DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1 mM; ecRecT 0.1 mM; NaCl 100 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM; pH: 7.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
transferred NOESYsample_1isotropicsample_conditions_1
DQF-COSYsample_1isotropicsample_conditions_1
TOCSYsample_1isotropicsample_conditions_1
ROESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAA14949 BAB35356 BAG76926 BAI25198 BAI25911
EMBL CAQ31853 CCP96881 CCP99639 CDJ71764 CDK47162
GB AAA16179 AAC74431 AAG56448 ABJ00798 ACB02570
REF NP_309960 NP_415865 WP_000166310 WP_000166311 WP_000166312
SP P33228
AlphaFold P33228