BMRB Entry 11144

Title:
Solution structure of the DnaJ domain from human Williams-Beuren syndrome chromosome region 18 protein
Deposition date:
2010-03-31
Original release date:
2011-04-01
Authors:
Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the DnaJ domain from human Williams-Beuren syndrome chromosome region 18 protein"  .

Assembly members:

Assembly members:
DnaJ domain, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050620-25

Data sets:
Data typeCount
13C chemical shifts390
15N chemical shifts91
1H chemical shifts586

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DnaJ domain1

Entities:

Entity 1, DnaJ domain 99 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERGLNGLY
2   ASPCYSSERTYRSERARGTHRALALEUTYR
3   ASPLEULEUGLYVALPROSERTHRALATHR
4   GLNALAGLNILELYSALAALATYRTYRARG
5   GLNCYSPHELEUTYRHISPROASPARGASN
6   SERGLYSERALAGLUALAALAGLUARGPHE
7   THRARGILESERGLNALATYRVALVALLEU
8   GLYSERALATHRLEUARGARGLYSTYRASP
9   ARGGLYLEULEUSERASPGLUASPLEUARG
10   GLYPROGLYSERGLYPROSERSERGLY

Samples:

sample_1: Gal-binding lectin, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9825, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB71671
GB AAM62307 AAS07471 EAW69658
REF NP_001181473 NP_115693 XP_001145797 XP_002817906 XP_003815619
SP Q96LL9
AlphaFold Q96LL9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks