BMRB Entry 11150

Title:
Solution structure of the Somatomedin B domain of human Ectonucleotide pyrophosphatase/phosphodiesterase family member
Deposition date:
2010-04-15
Original release date:
2011-05-05
Authors:
Abe, H.; Sasagawa, A.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Abe, H.; Sasagawa, A.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Somatomedin B domain of human Ectonucleotide pyrophosphatase/phosphodiesterase family member"  .

Assembly members:

Assembly members:
somatomedin B, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050118-03

Entity Sequences (FASTA):

Entity Sequences (FASTA):
somatomedin B: GSSGSSGWTCNKFRCGEKRL TRSLCACSDDCKDQGDCCIN YSSVCQGEKSSGPSSG

Data sets:
Data typeCount
13C chemical shifts172
15N chemical shifts47
1H chemical shifts263

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1somatomedin B1

Entities:

Entity 1, somatomedin B 56 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTRPTHRCYS
2   ASNLYSPHEARGCYSGLYGLULYSARGLEU
3   THRARGSERLEUCYSALACYSSERASPASP
4   CYSLYSASPGLNGLYASPCYSCYSILEASN
5   TYRSERSERVALCYSGLNGLYGLULYSSER
6   SERGLYPROSERSERGLY

Samples:

sample_1: somatomedin B, [U-13C; U-15N], 0.80 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
DBJ BAA02054 BAG37385
EMBL CAC39442
GB AAA63237 AAD38420 AAD38421 AAF36094 AAH59375
REF NP_001162404 NP_006199 XP_001103359 XP_002747012 XP_002817408
SP P22413
AlphaFold P22413

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks