BMRB Entry 11233

Title:
Solution structure of the SH3 domain of human KIAA1783 protein
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain of human KIAA1783 protein"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P051101-05

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts338
15N chemical shifts74
1H chemical shifts514

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 85 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERGLYTYR
2   VALILEALALEUARGSERTYRILETHRASP
3   ASNCYSSERLEULEUSERPHEHISARGGLY
4   ASPLEUILELYSLEULEUPROVALALATHR
5   LEUGLUPROGLYTRPGLNPHEGLYSERALA
6   GLYGLYARGSERGLYLEUPHEPROALAASP
7   ILEVALGLNPROALAALAALAPROASPPHE
8   SERPHESERLYSGLUGLNARGSERGLYSER
9   GLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.15 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

Delta v4.3, JEOL - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • JEOL ECA 700 MHz

Related Database Links:

PDB
DBJ BAB47412 BAG10147
GB EAW89288
REF NP_001296171 XP_001095714 XP_002827884 XP_004041092 XP_009431703
SP Q96JP2
AlphaFold Q96JP2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks