BMRB Entry 11254

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PDB ID: 1v6e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11254
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of a N-terminal Ubiquitin-like Domain in Mouse Tubulin-specific Chaperone B

Deposition date:

2010-08-09

Original release date:

2011-08-17

Authors:

Zhao, C.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Zhao, C.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of a N-terminal Ubiquitin-like Domain in Mouse Tubulin-specific Chaperone B" .

Assembly members:

Assembly members:
Ubiquitin-like domain, polymer, 95 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P030414-64

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubiquitin-like domain: GSSGSSGVMVFISSSLNSFR SEKRYSRSLTIAEFKCKLEL VVGSPASCMELELYGADDKF YSKLDQEDALLGSYPVDDGC RIHVIDHSGSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	402
15N chemical shifts	96
1H chemical shifts	614

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ubiquitin-like domain	1

Entities:

Entity 1, Ubiquitin-like domain 95 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

MET

VAL

2

PHE

ILE

SER

LEU

ASN

SER

PHE

ARG

3

SER

GLU

LYS

ARG

TYR

SER

ARG

SER

LEU

THR

4

ILE

ALA

GLU

PHE

LYS

CYS

LYS

LEU

GLU

LEU

5

VAL

GLY

SER

PRO

ALA

SER

CYS

MET

GLU

6

LEU

GLU

LEU

TYR

GLY

ALA

ASP

LYS

PHE

7

TYR

SER

LYS

LEU

ASP

GLN

GLU

ASP

ALA

LEU

8

LEU

GLY

SER

TYR

PRO

VAL

ASP

GLY

CYS

9

ARG

ILE

HIS

VAL

ILE

ASP

HIS

SER

GLY

SER

10

GLY

PRO

SER

GLY

Samples:

sample_1: Ubiquitin-like domain, [U-13C; U-15N], 1.15 mM; Phosphate Na 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B., A. - data analysis

Kujira v0.860, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1V6E
DBJ	BAB22009 BAB22918 BAB26939
GB	AAH10684 EDL24039 EDL24040 ELK17774
REF	NP_079824 XP_005361319 XP_006906006 XP_007096876 XP_011380142
SP	Q9D1E6
AlphaFold	Q9D1E6