BMRB Entry 11257

Title:
Solution Structure of Mouse Ubiquitin-like 3 Protein
Deposition date:
2010-08-09
Original release date:
2011-08-17
Authors:
Zhao, C.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Zhao, C.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of Mouse Ubiquitin-like 3 Protein"  .

Assembly members:

Assembly members:
Ubiquitin-like Fold, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P030414-75

Data sets:
Data typeCount
13C chemical shifts445
15N chemical shifts102
1H chemical shifts710

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubiquitin-like Fold1

Entities:

Entity 1, Ubiquitin-like Fold 116 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETSERSER
2   HISVALPROALAASPMETILEASNLEUARG
3   LEUILELEUVALSERGLYLYSTHRLYSGLU
4   PHELEUPHESERPROASNASPSERALASER
5   ASPILEALALYSHISVALTYRASPASNTRP
6   PROMETASPTRPGLUGLUGLUGLNVALSER
7   SERPROASNILELEUARGLEUILETYRGLN
8   GLYARGPHELEUHISGLYASNVALTHRLEU
9   GLYALALEULYSLEUPROPHEGLYLYSTHR
10   THRVALMETHISLEUVALALAARGGLUTHR
11   LEUPROGLUPROASNSERGLNGLYGLNARG
12   SERGLYPROSERSERGLY

Samples:

sample_1: Ubiquitin-like Fold mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8996, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB EMP31155 KFO80335
REF XP_009564834

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks