BMRB Entry 11550

Title:
Active-Site Structure of the Thermophilic Foc-Subunit Ring in Membranes Elucidated by Solid-State NMR
Deposition date:
2014-02-10
Original release date:
2017-08-03
Authors:
Kang, Su-Jin; Yasuto, Todokoro; Akustu, Hideo
Citation:

Citation: Kang, Su-Jin; Yasuto, Todokoro; Ikuko, Yumen; Bo, Shen; Iku, Iwasaki; Toshiharu, Suzuki; Atsushi, Miyagi; Masasuke, Yoshida; Toshimichi, Fujiwara; Akutsu, Hideo. "Active-site structure of the thermophilic foc-subunit ring in membranes elucidated by solid-state NMR"  Biophys J. 106, 390-398 (2014).
PubMed: 24461014

Assembly members:

Assembly members:
subunit c of thermophilic FoF1 ATP synthase, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus sp. PS3   Taxonomy ID: 2334   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: wheat germ - cell free   Vector: pZA01MCS-SP6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
subunit c of thermophilic FoF1 ATP synthase: MHLGVLAAAIAVGLGALGAG IGNGLIVSRTIEGIARQPEL RPVLQTTMFIGVALVEALPI IGVVFSFIYLGR

Data typeCount
13C chemical shifts19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Foc-subunit_11
2Foc-subunit_21
3Foc-subunit_31
4Foc-subunit_41
5Foc-subunit_51
6Foc-subunit_61
7Foc-subunit_71
8Foc-subunit_81
9Foc-subunit_91
10Foc-subunit_101

Entities:

Entity 1, Foc-subunit_1 72 residues - Formula weight is not available

1   METHISLEUGLYVALLEUALAALAALAILE
2   ALAVALGLYLEUGLYALALEUGLYALAGLY
3   ILEGLYASNGLYLEUILEVALSERARGTHR
4   ILEGLUGLYILEALAARGGLNPROGLULEU
5   ARGPROVALLEUGLNTHRTHRMETPHEILE
6   GLYVALALALEUVALGLUALALEUPROILE
7   ILEGLYVALVALPHESERPHEILETYRLEU
8   GLYARG

Samples:

sample_1: thermophilic Foc-subunit ring, [U-100% 13C; U-100% 15N; U-80% 2H], 2.8 mg; D2O 100%; Tris-DCl 10 mM

sample_conditions_1: ionic strength: 0.010 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DARRsample_1solidsample_conditions_1
DARRsample_1solidsample_conditions_1
Cai+1-(C'Ca) correlationsample_1solidsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian infinity-plus 700 MHz
  • Varian infinity-plus 600 MHz