BMRB Entry 15866

Title:
CIN85 Sh3-C domain in complex with ubiquitin
Deposition date:
2008-07-07
Original release date:
2008-10-08
Authors:
Forman-Kay, Julie; Bezsonova, Irina
Citation:

Citation: Bezsonova, Irina; Bruce, Christine; Wiesner, Silke; Lin, Hong; Rotin, Daniela; Forman-Kay, Julie. "Interactions between the three CIN85 SH3 domains and ubiquitin: implications for CIN85 ubiquitination."  Biochemistry 47, 8937-8949 (2008).
PubMed: 18680311

Assembly members:

Assembly members:
CIN85 Sh3-C domain, polymer, 62 residues, 7151.159 Da.
ubiquitin, polymer, 76 residues, 8623.001 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28-b

Data sets:
Data typeCount
13C chemical shifts116
15N chemical shifts57
1H chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1_11
2entity_2_12
3entity_1_21
4entity_2_22
5entity_1_31
6entity_2_32
7entity_1_41
8entity_2_42
9entity_1_51
10entity_2_52
11entity_1_61
12entity_2_62
13entity_1_71
14entity_2_72
15entity_1_81
16entity_2_82

Entities:

Entity 1, entity_1_1 62 residues - 7151.159 Da.

1   LYSSERLYSASPTYRCYSLYSVALILEPHE
2   PROTYRGLUALAGLNASNASPASPGLULEU
3   THRILELYSGLUGLYASPILEVALTHRLEU
4   ILEASNLYSASPCYSILEASPVALGLYTRP
5   TRPGLUGLYGLULEUASNGLYARGARGGLY
6   VALPHEPROASPASNPHEVALLYSLEULEU
7   PROPRO

Entity 2, entity_2_1 76 residues - 8623.001 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYCYS

Samples:

sample_2: entity_1 0.1-1.0 mM; entity_2, [U-100% 15N], 0.1-5.0 mM; sodium phosphate 50 mM; DTT 2 mM; DSS 5 mM; sodium chloride 150 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_1: entity_1, [U-100% 15N], 0.1-1.0 mM; entity_2 0.1-5.0 mM; sodium phosphate 50 mM; DTT 2 mM; DSS 5 mM; sodium chloride 150 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_3: entity_1, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 50 mM; DTT 2 mM; DSS 5 mM; sodium chloride 150 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.150 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
IPAPsample_1isotropicsample_conditions_1
IPAPsample_2isotropicsample_conditions_1

Software:

HADDOCK, Cyril Dominguez, Rolf Boelens and Alexandre M.J.J. Bonvin - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAE36553 BAH11471 BAH13234 BAA03983 BAA09860 BAA11842 BAA11843 BAA23486
GB AAK51627 AAK51628 AAK95587 AAL82459 AAL82460 AAA02769 AAA28154 AAA28997 AAA28998 AAA28999
REF NP_001129200 NP_001171889 XP_004000379 XP_004063940 XP_004401574 NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286
BMRB 11505 11547 15047 15410 15689 15907 16228 16582 16626 16763 16880 16885 16895 17059 17181 17239 17333 17439 17769 17919 18582 18583 18584 18610 18611 18737 19394 19399 19406 19412 19447 25070 25230 4245 4375 4983 5101 5387 6457 6466 6470 6488 68 7111
EMBL CAA26488 CAA28495 CAA30183 CAA30815 CAA33466
PIR I50437 I51568 I65237 JN0790 S13928
PRF 0412265A 1212243A 1212243B 1212243C 1212243D
SP P0C273 P0C275 P0C276 P0CG47 P0CG48
TPD FAA00319
TPG DAA18802 DAA20663 DAA20672 DAA24675 DAA28295
AlphaFold P0C275 P0C273 P0CG48 P0CG47 P0C276

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks