BMRB Entry 15936
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15936
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Title: Sequence-specific 1H, 15N, resonance assignments of nine 1H-{15N} HSQC spectras for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit recorded at TFE concentrations increasing in the range of 0-30% PubMed: 19847776
Authors: Kaczka, Piotr; Polkowska, Agnieszka
Citation: Kaczka, Piotr; Polkowska-Nowakowska, Agnieszka; Bolewska, Krystyna; Zhukov, Igor; Poznaski, Jarosaw; Wierzchowski, Kazimierz. "Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit." Proteins 78, 754-768 (2010).
Assembly members:
4_sigma70, polymer, 107 residues, 12032.5 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
4_sigma70: MGSSHHHHHHSSGLVPRGSH
MLELPLDSATTESLRAATHD
VLAGLTAREAKVLRMRFGID
MNTDYTLEEVGKQFDVTRER
IRQIEAKALRKLRHPSRSEV
LRSFLDD
| Data type | Count |
| 15N chemical shifts | 1000 |
| 1H chemical shifts | 1000 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 4 sigma70 | 1 |
Entities:
Entity 1, 4 sigma70 107 residues - 12032.5 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | LEU | GLU | LEU | PRO | LEU | ASP | SER | ALA | THR | ||||
| 4 | THR | GLU | SER | LEU | ARG | ALA | ALA | THR | HIS | ASP | ||||
| 5 | VAL | LEU | ALA | GLY | LEU | THR | ALA | ARG | GLU | ALA | ||||
| 6 | LYS | VAL | LEU | ARG | MET | ARG | PHE | GLY | ILE | ASP | ||||
| 7 | MET | ASN | THR | ASP | TYR | THR | LEU | GLU | GLU | VAL | ||||
| 8 | GLY | LYS | GLN | PHE | ASP | VAL | THR | ARG | GLU | ARG | ||||
| 9 | ILE | ARG | GLN | ILE | GLU | ALA | LYS | ALA | LEU | ARG | ||||
| 10 | LYS | LEU | ARG | HIS | PRO | SER | ARG | SER | GLU | VAL | ||||
| 11 | LEU | ARG | SER | PHE | LEU | ASP | ASP |
Samples:
sample_1: 4 sigma70, [U-13C; U-15N], 3 mM
sample_2: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 0.3%
sample_3: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 2%
sample_4: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 4%
sample_5: 4 of sigma70, [U-13C; U-15N], 3 mM; TFE 6%
sample_6: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 10%
sample_7: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 12%
sample_8: 4 sigma70 3 mM; TFE 15%
sample_9: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 20%
sample_10: 4 sigma70, [U-13C; U-15N], 3 mM; TFE 30%
sample_conditions_all: ionic strength: 0.0 M; pH: 4.55; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_4 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_5 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_6 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_7 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_8 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_9 | isotropic | sample_conditions_all |
| 2D 1H-15N HSQC | sample_10 | isotropic | sample_conditions_all |
Software:
NMRPipe v1.7, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.111, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Varian UnityPlus 500 MHz