BMRB Entry 16094

Title:
solution structure of anntoxin
Deposition date:
2008-12-29
Original release date:
2009-06-26
Authors:
Hong, Jing; You, Dewen; Lai, Ren; Lin, Donghai
Citation:

Citation: You, Dewen; Hong, Jing; Rong, Mingqiang; Yu, Haining; Liang, Songping; Ma, Yufang; Yang, Hailong; Wu, Jing; Lin, Donghai; Lai, Ren. "The first gene-encoded amphibian neurotoxin."  J. Biol. Chem. 284, 22079-22086 (2009).
PubMed: 19535333

Assembly members:

Assembly members:
anntoxin, polymer, 61 residues, 6834.479 Da.

Natural source:

Natural source:   Common Name: Assam treefrog   Taxonomy ID: 317325   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hyla annectans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET32a+

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts336

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1anntoxin1

Entities:

Entity 1, anntoxin 61 residues - 6834.479 Da.

1   PROALAGLNASPTYRARGCYSGLNLEUSER
2   ARGASNTYRGLYLYSGLYSERGLYSERPHE
3   THRASNTYRTYRTYRASPLYSALATHRSER
4   SERCYSLYSTHRPHEARGTYRARGGLYSER
5   GLYGLYASNGLYASNARGPHELYSTHRLEU
6   GLUASPCYSGLUALATHRCYSVALTHRALA
7   GLU

Samples:

sample_1: entity 2 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: entity 2 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB ACU21613