BMRB Entry 16499

Title:
(1-77)Apomyoglobin
Deposition date:
2009-09-15
Original release date:
2010-11-01
Authors:
Fedyukina, Daria; Cavagnero, Silvia
Citation:

Citation: Fedyukina, Daria; Rajagopalan, Senapathy; Sekhar, Ashok; Fulmer, Eric; Eun, Ye-Jin; Cavagnero, Silvia. "Contribution of long-range interactions to the secondary structure of an unfolded globin."  Biophys. J. 99, L37-L39 (2010).
PubMed: 20816043

Assembly members:

Assembly members:
1-77_Apomyoglobin, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Sperm Whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-17b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts227
15N chemical shifts76
1H chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1(1-77)Apomyoglobin1

Entities:

Entity 1, (1-77)Apomyoglobin 78 residues - Formula weight is not available

1   METVALLEUSERGLUGLYGLUTRPGLNLEU
2   VALLEUHISVALTRPALALYSVALGLUALA
3   ASPVALALAGLYHISGLYGLNASPILELEU
4   ILEARGLEUPHELYSSERHISPROGLUTHR
5   LEUGLULYSPHEASPARGPHELYSHISLEU
6   LYSTHRGLUALAGLUMETLYSALASERGLU
7   ASPLEULYSLYSHISGLYVALTHRVALLEU
8   THRALALEUGLYALAILELEULYS

Samples:

sample_1: (1-77)Apomyoglobin, [U-99% 13C; U-99% 15N], 313 uM; CD3COOH 5 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 2.40; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4676 4695
PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185
AlphaFold P02185

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks