BMRB Entry 16551

Title:
Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO
Deposition date:
2009-10-11
Original release date:
2010-02-18
Authors:
Merkulova, Maria; Bakulina, Anastasia; Thaker, Youg; Gr ber, Gerhard; Marshansky, Vladimir
Citation:

Citation: Merkulova, Maria; Bakulina, Anastasia; Thaker, Youg Raj; Gruber, Gerhard; Marshansky, Vladimir. "Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO."  Biochim. Biophys. Acta 1797, 1398-1409 (2010).
PubMed: 20153292

Assembly members:

Assembly members:
ARNO-p(375-400), polymer, 26 residues, 3083 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ARNO-p(375-400): VSVDPFYEMLAARKKRIXVK KKQEQP

Data sets:
Data typeCount
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ARNO-p(375-400)1

Entities:

Entity 1, ARNO-p(375-400) 26 residues - 3083 Da.

1   VALSERVALASPPROPHETYRGLUMETLEU
2   ALAALAARGLYSLYSARGILESEPVALLYS
3   LYSLYSGLNGLUGLNPRO

Samples:

sample_1: ARNO-p(375-400)1 – 2 mM; H20 90%; D20 10%

sample_conditions_1: temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

REF XP_006643234