BMRB Entry 17105

Title:
Solution Structure of the N-terminal Domain of NP_954075.1
Deposition date:
2010-08-05
Original release date:
2010-08-18
Authors:
Susac, Lukas; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt
Citation:

Citation: Susac, Lukas; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of the protein NP_954075.1 from Geobacter sulfurreducens"  .

Assembly members:

Assembly members:
NP_954075.1, polymer, 109 residues, 12045.543 Da.

Natural source:

Natural source:   Common Name: d-proteobacteria   Taxonomy ID: 35554   Superkingdom: bacteria   Kingdom: not available   Genus/species: Geobacter sulfurreducens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSpeedET

Data sets:
Data typeCount
13C chemical shifts353
15N chemical shifts113
1H chemical shifts732

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NP_954075.11

Entities:

Entity 1, NP_954075.1 109 residues - 12045.543 Da.

1   GLYMETSERALAASPGLYSERGLUTYRGLY
2   ARGTYRPHEGLUGLNLEUGLNLYSVALASN
3   LEUTHRVALARGLEUGLYASPTHRGLYSER
4   PHEASPGLYTHRALAALAILETHRSERLEU
5   LYSGLYSERLEUALATRPLEUGLULEUPHE
6   GLYALAGLUGLNPROPROPROASNTHRLEU
7   SERGLUGLYALAGLUVALSERVALSERVAL
8   TRPTHRGLYGLYALALEUCYSARGCYSASP
9   GLYARGVALGLUTHRLEUARGASPASPARG
10   GLNPHEALAILEARGLEUVALGLYARGVAL
11   ARGGLULEUGLNARGARGGLUTYRPHE

Samples:

sample_1: NP_954075.1, [U-95% 13C; U-95% 15N], 0.9 mM; sodium phosphate 25 mM; sodium azide 4.5 mM; H2O 95%; H2O 5%

sample_conditions_1: ionic strength: 0.075 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY (ali)sample_1isotropicsample_conditions_1
4D APSY-HACANHsample_1isotropicsample_conditions_1
5D APSY-HACA(CO)NHsample_1isotropicsample_conditions_1
5D APSY-CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY (aro)sample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

TOPSPIN, Bruker Biospin - collection, processing

UNIO, Hermann and Wuthrich - chemical shift assignment, peak picking, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAR36425 ADI85785 AJY69279
REF NP_954075 WP_010943660 WP_014552065

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks