BMRB Entry 17345

Title:
Solution NMR structure of human NFATC2IP ubiquitin-like domain, NFATC2IP_244_338, NESG target HT65A/OCSP target hs00387_244_338/SGC-toronto
Deposition date:
2010-12-03
Original release date:
2011-01-05
Authors:
Wu, Bin; Yee, Adelinda; Houliston, Scott; Doherty, Ryan; Semesi, Anthony; Dhe-Paganon, Sirano; Arrowsmith, Cheryl
Citation:

Citation: Doherty, Ryan; Wu, Bin; Yee, Adelinda; Lemak, Alexander; Houliston, Scott; Fares, Christophe; Srisalam, Sampath; Bulter, C; Edwards, Aled; Weigelt, Johan; Dhe-Paganon, Sirano; Arrowsmith, Cheryl. "Human NFATC2IP ubiquitin-like domains"  .

Assembly members:

Assembly members:
NFATC2IP_244_338, polymer, 95 residues, 10396.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p15Tv lic

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts414
15N chemical shifts87
1H chemical shifts693

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NFATC2IP_244_3381

Entities:

Entity 1, NFATC2IP_244_338 95 residues - 10396.201 Da.

1   GLNGLYGLNGLUASPGLUVALVALLEUVAL
2   GLUGLYPROTHRLEUPROGLUTHRPROARG
3   LEUPHEPROLEULYSILEARGCYSARGALA
4   ASPLEUVALARGLEUPROLEUARGMETSER
5   GLUPROLEUGLNSERVALVALASPHISMET
6   ALATHRHISLEUGLYVALSERPROSERARG
7   ILELEULEULEUPHEGLYGLUTHRGLULEU
8   SERPROTHRALATHRPROARGTHRLEULYS
9   LEUGLYVALALAASPILEILEASPCYSVAL
10   VALLEUTHRSERSER

Samples:

sample_1: NFATC2IP_244_338, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; zinc sulphate 10 uM; DTT, [U-100% 2H], 10 mM; NaN3 0.01%; benzamidine 10 mM; inhibitor cocktail 1 x; H2O 90%; D2O 10%

sample_2: NFATC2IP_244_338, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS, [U-100% 2H], 10 mM; sodium chloride 300 mM; zinc sulphate 10 uM; DTT, [U-100% 2H], 10 mM; NaN3 0.01%; benzamidine 10 mM; inhibitor cocktail 1 x; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

MDDGUI v1.0, Gutmanas, Arrowsmith - processing

SPARKY v3.95, Goddard - data analysis

FMCGUI v2.4, Lemak, Arrowsmith - chemical shift assignment

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

AutoStruct, Huang, Tejero, Powers and Montelione - NMR structure quality assessment

PSVS, Bhattacharya and Montelione - NMR structure quality assessment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAC11189
GB AAH18311 AAH21551 AAH80628 AAI01742 AAI12183
REF NP_116204 XP_001146533 XP_002826318 XP_003804645 XP_004057462
SP Q8NCF5
AlphaFold Q8NCF5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks