BMRB Entry 17405

Title:
IPSE/alpha-1
Deposition date:
2011-01-11
Original release date:
2011-05-03
Authors:
Meyer, N. Helge; Sattler, Michael
Citation:

Citation: Meyer, N. Helge; Schramm, Gabriele; Sattler, Michael. "1H, 13C and 15N chemical shift assignments of IPSENLS."  Biomol. NMR Assignments 5, 225-227 (2011).
PubMed: 21516468

Assembly members:

Assembly members:
IPSE/alpha-1, polymer, 106 residues, 11967 Da.

Natural source:

Natural source:   Common Name: Flatworm   Taxonomy ID: 6183   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schistosoma mansoni

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet

Data sets:
Data typeCount
13C chemical shifts475
15N chemical shifts102
1H chemical shifts716

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IPSE1

Entities:

Entity 1, IPSE 106 residues - 11967 Da.

1   GLYALAASPSERCYSLYSTYRCYSLEUGLN
2   LEUTYRASPGLUTHRTYRGLUARGGLYSER
3   TYRILEGLUVALTYRLYSSERVALGLYSER
4   LEUSERPROPROTRPTHRPROGLYSERVAL
5   CYSVALPROPHEVALASNASPTHRLYSARG
6   GLUARGPROTYRTRPTYRLEUPHEASPASN
7   VALASNTYRTHRGLYARGILETHRGLYLEU
8   GLYHISGLYTHRCYSILEASPASPPHETHR
9   LYSSERGLYPHELYSGLYILESERSERILE
10   LYSARGCYSILEGLNTHRLYSASPGLYLYS
11   VALGLUCYSILEASNGLN

Samples:

sample_1: IPSE/alpha-1, [U-13C; U-15N], 1 mM; IPSE/alpha-1, [U-10% 13C], 1 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III 750 MHz
  • Bruker Avance III 900 MHz

Related Database Links:

PDB
EMBL CCD78463
GB AAK26170 AAZ85122

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks