BMRB Entry 17533

Title:
Solution structure of the Sex Peptide from Drosophila melanogaster
Deposition date:
2011-03-17
Original release date:
2011-05-03
Authors:
Moehle, Kerstin; Freund, Annabelle; Robinson, John
Citation:

Citation: Moehle, Kerstin; Freund, Annabelle; Kubli, Eric; Robinson, John. "NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster."  FEBS Lett. 585, 1197-1202 (2011).
PubMed: 21439282

Assembly members:

Assembly members:
entity, polymer, 36 residues, 4435.077 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: WEWPWNRKXTKFXIXSXNXR DKWCRLNLGPAWGGRC

Data sets:
Data typeCount
13C chemical shifts77
15N chemical shifts36
1H chemical shifts251

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sex Peptide from Drosophila melanogaster1

Entities:

Entity 1, Sex Peptide from Drosophila melanogaster 36 residues - 4435.077 Da.

1   TRPGLUTRPPROTRPASNARGLYSHYPTHR
2   LYSPHEHYPILEHYPSERHYPASNHYPARG
3   ASPLYSTRPCYSARGLEUASNLEUGLYPRO
4   ALATRPGLYGLYARGCYS

Samples:

sample_1: TSP0.7 – 3 mM; H2O 90%; D2O 10%

sample_2: TSP0.7 – 3 mM; D2O 100%

sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection, processing

DYANA, Guntert, Braun and Wuthrich - refinement, structure solution

SPSCAN, Glaser - peak integration

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks