BMRB Entry 17599

Title:
Solution structure of Par-6 Q144C/L164C
Deposition date:
2011-04-25
Original release date:
2011-12-01
Authors:
Volkman, B.; Whitney, D.; Peterson, F.
Citation:

Citation: Whitney, Dustin; Peterson, Francis; Volkman, Brian. "A Conformational Switch in the CRIB-PDZ Module of Par-6"  Structure 19, 1711-1722 (2011).
PubMed: 22078569

Assembly members:

Assembly members:
Par-6, polymer, 128 residues, 13724.863 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBH4

Data sets:
Data typeCount
13C chemical shifts529
15N chemical shifts123
1H chemical shifts869

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Par-61

Entities:

Entity 1, Par-6 128 residues - 13724.863 Da.

The N-terminal GS dipeptide is a cloning artifact.

1   GLYSERLYSTHRLYSALAPROSERILESER
2   ILEPROHISASPPHEARGCYSVALSERALA
3   ILEILEASPVALASPILEVALPROGLUTHR
4   HISARGARGVALARGLEUCYSLYSHISGLY
5   SERASPLYSPROLEUGLYPHETYRILEARG
6   ASPGLYTHRSERVALARGVALTHRALASER
7   GLYLEUGLULYSGLNPROGLYILEPHEILE
8   SERARGLEUVALPROGLYGLYLEUALAGLU
9   SERTHRGLYLEULEUALAVALASNASPGLU
10   VALILEGLUVALASNGLYILEGLUVALALA
11   GLYLYSTHRLEUASPGLNVALTHRASPMET
12   METVALALAASNSERSERASNLEUILEILE
13   THRVALLYSPROALAASNGLNARG

Samples:

sample_1: Par-6, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 53 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

  • Bruker Avance II 600 MHz

Related Database Links:

BMRB 17600
PDB
GB AAD15927 AAF48757 AAL28917 AAN09592 ACL90845
REF NP_573238 NP_728094 XP_001963708 XP_001977590 XP_001991396

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks