BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18041

Title: Solution structure of the EDC3-LSm domain   PubMed: 22085934

Authors: Truffault, Vincent

Citation: Fromm, Simon; Truffault, Vincent; Kamenz, Julia; Braun, Joerg; Hoffmann, Niklas; Izaurralde, Elisa; Sprangers, Remco. "The structural basis of Edc3- and Scd6-mediated activation of the Dcp1:Dcp2 mRNA decapping complex."  EMBO J. 31, 279-290 (2011).

Assembly members:
EDC3-LSm, polymer, 125 residues, 14016.833 Da.

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
EDC3-LSm: GAMGMSVADFYGSNVEVLLN NDSKARGVITNFDSSNSILQ LRLANDSTKSIVTKDIKDLR ILPKNEIMPKNGTKSPSTNS TKLKSAETYSSKNKWSMDCD EEFDFAANLEKFDKKQVFAE FREKD

Data sets:
Data typeCount
13C chemical shifts386
15N chemical shifts93
1H chemical shifts596

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EDC3-LSm domain1

Entities:

Entity 1, EDC3-LSm domain 125 residues - 14016.833 Da.

1   GLYALAMETGLYMETSERVALALAASPPHE
2   TYRGLYSERASNVALGLUVALLEULEUASN
3   ASNASPSERLYSALAARGGLYVALILETHR
4   ASNPHEASPSERSERASNSERILELEUGLN
5   LEUARGLEUALAASNASPSERTHRLYSSER
6   ILEVALTHRLYSASPILELYSASPLEUARG
7   ILELEUPROLYSASNGLUILEMETPROLYS
8   ASNGLYTHRLYSSERPROSERTHRASNSER
9   THRLYSLEULYSSERALAGLUTHRTYRSER
10   SERLYSASNLYSTRPSERMETASPCYSASP
11   GLUGLUPHEASPPHEALAALAASNLEUGLU
12   LYSPHEASPLYSLYSGLNVALPHEALAGLU
13   PHEARGGLULYSASP

Samples:

sample_1: EDC3-LSm, [U-100% 15N], 0.7 mM; NaPO4 50 mM; imidazole 10 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18042
PDB
EMBL CAA22671
REF NP_595858
SP O94752