BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18043

Title: The Insect Defensin Lucifensin from Lucilia sericata   PubMed: 22322867

Authors: Nygaard, Mads; Andersen, Anders; Kristensen, Hans-Henrik; Krogfelt, Karen; Fojan, Peter; Wimmer, Reinhard

Citation: Nygaard, Mads; Andersen, Anders; Kristensen, Hans-Henrik; Krogfelt, Karen; Fojan, Peter; Wimmer, Reinhard. "The insect defensin lucifensin from Lucilia sericata"  J. Biomol. NMR 52, 277-282 (2012).

Assembly members:
lucifensin, polymer, 40 residues, 4129.809 Da.

Natural source:   Common Name: Sheep blowfly   Taxonomy ID: 13632   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lucilia sericata

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
lucifensin: ATCDLLSGTGVKHSACAAHC LLRGNRGGYCNGRAICVCRN

Data sets:
Data typeCount
13C chemical shifts92
1H chemical shifts238

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lucifensin1

Entities:

Entity 1, lucifensin 40 residues - 4129.809 Da.

1   ALATHRCYSASPLEULEUSERGLYTHRGLY
2   VALLYSHISSERALACYSALAALAHISCYS
3   LEULEUARGGLYASNARGGLYGLYTYRCYS
4   ASNGLYARGALAILECYSVALCYSARGASN

Samples:

lucifensin_pH5: lucifensin 2 mM; potassium phosphate 10 mM; D2O 5%; TSP 15 uM; H2O 95%

pH5_25dg: pH: 5.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYlucifensin_pH5isotropicpH5_25dg
2D 1H-1H NOESYlucifensin_pH5isotropicpH5_25dg
2D DQF-COSYlucifensin_pH5isotropicpH5_25dg
2D 1H-13C HSQClucifensin_pH5isotropicpH5_25dg
2D 1H-13C HSQC aromaticlucifensin_pH5isotropicpH5_25dg

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - geometry optimization, structure solution

TALOS+, Cornilescu, Delaglio and Bax - geometry optimization

YASARA v11.5.21, Elmar Krieger - refinement

ProcheckNMR, Laskowski and MacArthur - structure validation

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB ADI87383 KNC22294
SP B3EWY5 P86471