BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18110

Title: Solution Structure of C-terminal RAGE (ctRAGE)   PubMed: 2194616

Authors: Rai, Vivek; Maldonado, Andres; Burz, David; Reverdatto, Sergey; Schmidt, Ann Marie; Shekhtman, Alexander

Citation: Sinclair, A.; Barnett, A.; Lunec, J.. "Free radicals and antioxidant systems in health and disease."  Br. J. Hosp. Med. 43, 334-344 (1990).

Assembly members:
ctRAGE, polymer, 43 residues, 1990.314 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ctRAGE: MWQRRQRRGEERKAPENQEE EEERAELNQSEEPEAGESST GGP

Data sets:
Data typeCount
13C chemical shifts107
15N chemical shifts40
1H chemical shifts226

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal RAGE (ctRAGE)1

Entities:

Entity 1, C-terminal RAGE (ctRAGE) 43 residues - 1990.314 Da.

1   METTRPGLNARGARGGLNARGARGGLYGLU
2   GLUARGLYSALAPROGLUASNGLNGLUGLU
3   GLUGLUGLUARGALAGLULEUASNGLNSER
4   GLUGLUPROGLUALAGLYGLUSERSERTHR
5   GLYGLYPRO

Samples:

sample_1: ctRAGE, [U-99% 13C; U-99% 15N], 300 uM; D2O, [U-100% 2H], 10%; potassium phosphate 10 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 90%

sample_conditions_1: ionic strength: 130 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

DBJ BAA05958 BAA89369 BAC65466 BAG35995 BAG60385
EMBL CAB43094
GB AAA03574 AAB47491 AAH20669 AAX07272 AAX07274
REF NP_001127 NP_001193858 NP_001193861
SP Q15109