BMRB Entry 18183

Title:
Identification and structural basis for a novel interaction between Vav2 and Arap3
Deposition date:
2012-01-05
Original release date:
2012-11-19
Authors:
Wu, Bo; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu
Citation:

Citation: Wu, Bo; Wang, Fengsong; Zhang, Jiahai; Zhang, Zhiyong; Qin, Liying; Peng, Junhui; Li, Fudong; Liu, Jianping; Lu, Guowei; Gong, Qingguo; Yao, Xuebiao; Wu, Jihui; Shi, Yunyu. "Identification and structural basis for a novel interaction between Vav2 and Arap3"  J. Struct. Biol. 180, 84-95 (2012).
PubMed: 22750419

Assembly members:

Assembly members:
entity, polymer, 113 residues, 13480.319 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts431
15N chemical shifts116
1H chemical shifts821

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Vav21

Entities:

Entity 1, Vav2 113 residues - 13480.319 Da.

1   SERARGPROPROSERARGGLUILEASPTYR
2   THRALATYRPROTRPPHEALAGLYASNMET
3   GLUARGGLNGLNTHRASPASNLEULEULYS
4   SERHISALASERGLYTHRTYRLEUILEARG
5   GLUARGPROALAGLUALAGLUARGPHEALA
6   ILESERILELYSPHEASNASPGLUVALLYS
7   HISILELYSVALVALGLULYSASPASNTRP
8   ILEHISILETHRGLUALALYSLYSPHEASP
9   SERLEULEUGLULEUVALGLUTYRTYRGLN
10   CYSHISSERLEULYSGLUSERPHELYSGLN
11   LEUASPTHRTHRLEULYSTYRPROTYRLYS
12   SERARGGLU

Samples:

sample_1: entity, [U-13C; U-15N], 1 mM; DTT 5 mM; sodium chloride 75 mM; potassium phosphate 5 mM; sodium phosphate 20 mM; EDTA 1 mM; H2O 90%; D2O 10%

sample_2: entity, [U-13C; U-15N], 1 mM; sodium chloride 75 mM; DTT 5 mM; potassium phosphate 5 mM; sodium phosphate 20 mM; EDTA 1 mM; D2O 100%

Sample_condition_1: ionic strength: 100 mM; pH: 6.2; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicSample_condition_1
3D CBCA(CO)NHsample_1isotropicSample_condition_1
3D HNCACBsample_1isotropicSample_condition_1
3D HNCOsample_1isotropicSample_condition_1
3D HCACOsample_1isotropicSample_condition_1
3D C(CO)NHsample_1isotropicSample_condition_1
3D H(CCO)NHsample_1isotropicSample_condition_1
3D HBHA(CO)NHsample_1isotropicSample_condition_1
3D 1H-15N NOESYsample_1isotropicSample_condition_1
3D HCCH-TOCSYsample_2isotropicSample_condition_1
3D HCCH-COSYsample_2isotropicSample_condition_1
3D 1H-13C NOESYsample_2isotropicSample_condition_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks