BMRB Entry 18240

Title:
Mutant of the sub-genomic promoter from Brome Mosaic Virus
Deposition date:
2012-02-06
Original release date:
2012-03-13
Authors:
Skov, Joan
Citation:

Citation: Skov, Joan; Gaudin, Mathieu; Podbevsek, Peter; Olsthoorn, Rene; Petersen, Michael. "The subgenomic promoter of brome mosaic virus folds into a stem-loop structure capped by a pseudo-triloop that is structurally similar to the triloop of the genomic promoter."  RNA 18, 992-1000 (2012).
PubMed: 22393035

Assembly members:

Assembly members:
RNA_(5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3')_, polymer, 15 residues, 4792.932 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_(5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3')_: GAGGACAUAGUCUUC

Data sets:
Data typeCount
1H chemical shifts100
31P chemical shifts14

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3')1

Entities:

Entity 1, RNA (5'-R(*GP*AP*GP*GP*AP*CP*AP*UP*AP*GP*UP*CP*UP*UP*C)-3') 15 residues - 4792.932 Da.

1   GAGGACAUAG
2   UCUUC

Samples:

sample_1: delA_RNA 2 mM; sodium phosphate 10 mM; sodium chloride 50 mM; EDTA 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 65 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

X-PLOR v3.851, Brunger - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz