BMRB Entry 18953

Title:
Solution Structure of gammaM7-Crystallin
Deposition date:
2013-01-16
Original release date:
2013-06-04
Authors:
Mahler, Bryon; Wu, Zhengrong
Citation:

Citation: Mahler, Bryon; Chen, Yingwei; Ford, Jason; Thiel, Caleb; Wistow, Graeme; Wu, Zhengrong. "Structure and Dynamics of the Fish Eye Lens Protein, M7-Crystallin."  Biochemistry 52, 3579-3587 (2013).
PubMed: 23597261

Assembly members:

Assembly members:
entity, polymer, 174 residues, 20937.965 Da.

Natural source:

Natural source:   Common Name: Zebrafish   Taxonomy ID: 7955   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Danio rerio

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
13C chemical shifts648
15N chemical shifts165
1H chemical shifts817

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gammaM-crystallin1

Entities:

Entity 1, gammaM-crystallin 174 residues - 20937.965 Da.

1   METGLYLYSILEILEPHETYRGLUASPARG
2   ASNPHEGLYGLYARGTYRHISGLUCYSMET
3   SERASPCYSALAASPLEUHISSERTYRPHE
4   ASNARGCYSHISSERILEARGVALGLUSER
5   GLYCYSPHEMETVALTYRASPARGTHRASN
6   PHEMETGLYARGGLNTYRPHELEUARGARG
7   GLYGLUTYRPROASPTYRMETARGTHRMET
8   GLYMETASNASPCYSVALARGSERCYSARG
9   METILEPROLEUHISHISGLYSERPHELYS
10   METARGLEUTYRGLUHISSERASPMETGLY
11   GLYARGMETMETGLULEUMETASPASPCYS
12   PROASNLEUMETASPARGPHEASNMETSER
13   ASPPHEHISSERCYSHISVALMETASPGLY
14   HISTRPLEUVALTYRGLUGLNPROASNTYR
15   THRGLYARGGLNPHETYRLEUARGPROGLY
16   GLUTYRARGSERTYRASNASPTRPGLYGLY
17   VALTHRSERARGMETGLYSERILEARGARG
18   ILETHRASPLEU

Samples:

sample_1: imidazole, [U-2H], 25 mM; potassium chloride 20 mM; DTT 2 mM; sodium azide 0.04%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.2; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-edited NOESY-HSQCsample_1isotropicsample_conditions_1
3D 13C-edited HMQC-NOESY-HMQCsample_1isotropicsample_conditions_1
RDC by HSQC-TROSYsample_1anisotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

AQUA, Rullmann, Doreleijers and Kaptein - refinement

ProcheckNMR, Laskowski and MacArthur - refinement

TALOS, Cornilescu, Delaglio and Bax - data analysis

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAI62654 AAI62659 AAU85784
REF NP_001018631

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks