BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19104

Title: Induced folding in RNA recognition by Arabidopsis thaliana DCL1   PubMed: 26101256

Authors: Suarez, Irina; Burdisso, Paula; Benoit, Matthieu; Boisbouvier, Jerome; Rasia, Rodolfo

Citation: Suarez, Irina; Burdisso, Paula; Benoit, Matthieu; Boisbouvier, Jerome; Rasia, Rodolfo. "Induced folding in RNA recognition by Arabidopsis thaliana DCL1"  Nucleic Acids Res. 43, 6607-6619 (2015).

Assembly members:
NB22, polymer, 83 residues, 9160.3879 Da.

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NB22: GHMMHPVRELQERCQQQAEG LEYKASRSGNTATVEVFIDG VQVGVAQNPQKKMAQKLAAR NALAALKEKEIAESKEKHIN NGN

Data sets:
Data typeCount
13C chemical shifts225
15N chemical shifts74
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NB22_11
2NB22_21
3NB22_31

Entities:

Entity 1, NB22_1 83 residues - 9160.3879 Da.

1   GLYHISMETMETHISPROVALARGGLULEU
2   GLNGLUARGCYSGLNGLNGLNALAGLUGLY
3   LEUGLUTYRLYSALASERARGSERGLYASN
4   THRALATHRVALGLUVALPHEILEASPGLY
5   VALGLNVALGLYVALALAGLNASNPROGLN
6   LYSLYSMETALAGLNLYSLEUALAALAARG
7   ASNALALEUALAALALEULYSGLULYSGLU
8   ILEALAGLUSERLYSGLULYSHISILEASN
9   ASNGLYASN

Samples:

sample_1: NB22, [U-13C; U-15N; U-2H], 400 uM; miR172ls 500 uM; sodium phosphate 20 mM; TCEP 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 20 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
HNCoCACB_NB22_RNA (H[N[co[{CA|ca[C]}]]])sample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.1, CCPN - secuential assignment

NMR spectrometers:

  • Varian DirectDrive 600 MHz

Related Database Links:

BMRB 19105
DBJ BAD94606
GB AAF03534 AAF26461 AAG38019 AAG38020 AEE27220
REF NP_001184881 NP_171612 XP_006306578 XP_006418369
SP Q9SP32