BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19484

Title: NMR structure of a two-transmembrane segment TM VI-VII of NHE1   PubMed: 24840010

Authors: Lee, Brian; Sykes, Brian; Alves, Claudia; Fliegel, Larry

Citation: Alves, Claudia; Lee, Brian; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of the Transmembrane Segment Pair VI and VII of the NHE1 Isoform of the Na(+)/H(+) Exchanger"  Biochemistry 53, 3658-3670 (2014).

Assembly members:
entity, polymer, 57 residues, 6257.431 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GSKKKDNLLFGSIISAVDPV AVLAVFEEIHINELLHILVF GESLLNDAVTVVLYKKK

Data sets:
Data typeCount
1H chemical shifts393

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tm671

Entities:

Entity 1, tm67 57 residues - 6257.431 Da.

1   GLYSERLYSLYSLYSASPASNLEULEUPHE
2   GLYSERILEILESERALAVALASPPROVAL
3   ALAVALLEUALAVALPHEGLUGLUILEHIS
4   ILEASNGLULEULEUHISILELEUVALPHE
5   GLYGLUSERLEULEUASNASPALAVALTHR
6   VALVALLEUTYRLYSLYSLYS

Samples:

sample_1: entity, [U-15N], 1 – 2 mM; D2O, [U-2H], 5%; DSS, [1,1,2,2,3,3-2H6], 0.25 mM; H2O 95%; dodecylphosphocholine, [U-2H], 75 – 150 mM

sample_2: entity, [U-15N], 1 – 2 mM; D2O, [U-2H], 100%; DSS, [1,1,2,2,3,3-2H6], 0.25 mM; dodecylphosphocholine, [U-2H], 75 – 150 mM

sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAE02258 BAE29332 BAE32882 BAE38221 BAE75800
EMBL CAA42558 CAA43721 CAA69925 CAB45232 CAF94405
GB AAA31092 AAA91483 AAA92976 AAA98479 AAB20633
REF NP_001007104 NP_001038108 NP_001081553 NP_001095191 NP_001273957
SP P19634 P23791 P26431 P48762 Q28036
TPG DAA32066