BMRB Entry 19628

Title:
NMR structure of the protein NP_419126.1 from CAULOBACTER CRESCENTUS
Deposition date:
2013-11-21
Original release date:
2014-03-03
Authors:
Serrano, Pedro; Dutta, Samit; Geralt, Michael; Wuthrich, Kurt
Citation:

Citation: Serrano, Pedro; Dutta, Samit; Geralt, Michael; Wuthrich, Kurt. "NMR structure of the protein NP_419126.1 from CAULOBACTER CRESCENTUS"  .

Assembly members:

Assembly members:
entity, polymer, 74 residues, 16470.863 Da.

Natural source:

Natural source:   Common Name: Caulobacter vibrioides   Taxonomy ID: 155892   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Caulobacter crescentus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSpeedET

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts302
15N chemical shifts74
1H chemical shifts491

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 74 residues - 16470.863 Da.

1   METPROASPLYSGLNLEULEUHISILEVAL
2   VALGLYGLYGLULEULYSASPVALALAGLY
3   VALGLUPHEARGASPLEUSERLYSVALGLU
4   PHEVALGLYALATYRPROSERTYRASPGLU
5   ALAHISLYSALATRPLYSALALYSALAGLN
6   ALATHRVALASPASNALAHISALAARGTYR
7   PHEILEILEHISALAHISLYSLEULEUASP
8   PROSERGLUGLY

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; entity, [U-99% 13C; U-99% 15N], 1.2 mM

sample_conditions_1: ionic strength: 0.0798 M; pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
4D HACANH APSYsample_1isotropicsample_conditions_1
5D HACACONH APSYsample_1isotropicsample_conditions_1
5D CBCACONH APSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al., Keller and Wuthrich - chemical shift assignment, data analysis, refinement

j-UNIO, Herrmann and Wuthrich - chemical shift assignment, chemical shift calculation, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAK22294 ACL93776
REF NP_419126 WP_010918196 WP_012639934 YP_002515684

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks