BMRB Entry 19712

Title:
Designed Exendin-4 analogues
Deposition date:
2013-12-30
Original release date:
2014-06-02
Authors:
Rovo, Petra; Farkas, Viktor; Straner, Pal; Jermendy, Agnes; Hegyi, Orsolya; Toth, Gabor; Perczel, Andras
Citation:

Citation: Rovo, Petra; Farkas, Viktor; Straner, Pal; Szabo, Maria; Jermendy, Agnes; Hegyi, Orsolya; Toth, Gabor; Perczel, Andras. "Rational Design of -Helix-Stabilized Exendin-4 Analogues."  Biochemistry 53, 3540-3552 (2014).
PubMed: 24828921

Assembly members:

Assembly members:
E19, polymer, 37 residues, 4307.771 Da.

Natural source:

Natural source:   Common Name: Gila monster   Taxonomy ID: 8554   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Heloderma suspectum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUBK2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E19: EGTFTSDLSKQMEEEAVRLY IQWLKEGGPSSGRPPPS

Data sets:
Data typeCount
1H chemical shifts250

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1E191

Entities:

Entity 1, E19 37 residues - 4307.771 Da.

1   GLUGLYTHRPHETHRSERASPLEUSERLYS
2   GLNMETGLUGLUGLUALAVALARGLEUTYR
3   ILEGLNTRPLEULYSGLUGLYGLYPROSER
4   SERGLYARGPROPROPROSER

Samples:

sample: E19 0.5-1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 300 K; viscosity: 1.002 cP

Experiments:

NameSampleSample stateSample conditions
TOCSYsampleisotropicsample_conditions_1
NOESYsampleisotropicsample_conditions_1
COSYsampleisotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Bruker DRX 700 MHz

Related Database Links:

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