BMRB Entry 20003

Title:
NMR Structure of Leucine-Enkephalin in Water
Deposition date:
2007-12-27
Original release date:
2008-05-27
Authors:
Mukhopadhyay, Chaitali; Gayen, Anindita
Citation:

Citation: Gayen, Anindita; Mukhopadhyay, Chaitali. "Evidence for Effect of GM1 on Opioid Peptide Conformation: NMR Study on Leucine Enkephalin in Ganglioside-Containing Isotropic Phospholipid Bicelles"  Langmuir 24, 5422-5432 (2008).
PubMed: 18412380

Assembly members:

Assembly members:
Leu-enkephalin, polymer, 5 residues, 555.62 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Leu-enkephalin: YGGFL

Data sets:
Data typeCount
13C chemical shifts24
1H chemical shifts28
coupling constants2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Leu-enkephalin1

Entities:

Entity 1, Leu-enkephalin 5 residues - 555.62 Da.

TYROSINE 1 GLYCINE 2 GLYCINE 3 PHENYLALANINE 4 LEUCINE 5

1   TYRGLYGLYPHELEU

Samples:

leucine_enkephalin: Leu-enkephalin 4-5 ± 0 mM; H2O 90%; D2O 10%

solution_in_90%_H2O_10%_D2O_pH_6.6_298K: pH: 6.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYleucine_enkephalinisotropicsolution_in_90%_H2O_10%_D2O_pH_6.6_298K
2D 1H-1H NOESYleucine_enkephalinisotropicsolution_in_90%_H2O_10%_D2O_pH_6.6_298K
2D DQF-COSYleucine_enkephalinisotropicsolution_in_90%_H2O_10%_D2O_pH_6.6_298K
2D 1H-13C HSQCleucine_enkephalinisotropicsolution_in_90%_H2O_10%_D2O_pH_6.6_298K

Software:

xwinnmr v3.75, Bruker Biospin, Goddard, Schwieters, Kuszewski, Tjandra and Clore - collection, data analysis, refinement

NMR spectrometers:

  • Bruker DRX 500 MHz