BMRB Entry 25024

Title:
3D structure of RP domain of MiSp
Deposition date:
2014-06-16
Original release date:
2015-06-15
Authors:
Yang, Daiwen; Gao, Zhenwei
Citation:

Citation: Gao, Daiwen; Yang, Daiwen. "3D structure of RP domain of MiSp"  .

Assembly members:

Assembly members:
entity, polymer, 125 residues, 12115.084 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-M

Data sets:
Data typeCount
13C chemical shifts136
15N chemical shifts112
1H chemical shifts263

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RP domain of MiSp1

Entities:

Entity 1, RP domain of MiSp 125 residues - 12115.084 Da.

1   GLYASNALAPHEALAGLNSERLEUSERSER
2   ASNLEULEUSERSERGLYASPPHEVALGLN
3   METILESERSERTHRTHRSERTHRASPGLN
4   ALAVALSERVALALATHRSERVALALAGLN
5   ASNVALGLYASNGLNLEUGLYLEUASPALA
6   ASNALAMETASNSERLEULEUGLYALAVAL
7   SERGLYTYRVALSERTHRLEUGLYASNALA
8   ILESERASPALASERALATYRALAASNALA
9   ILESERSERALAILEGLYASNVALLEUALA
10   ASNSERGLYSERILESERGLUSERTHRALA
11   SERSERALAALASERSERALAALASERSER
12   VALTHRTHRTHRLEUTHRSERTYRGLYPRO
13   ALAVALPHETYRALA

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.6 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.3 M; pH: 7; pressure: 1 atm; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB ABC72645

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks