BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25229

Title: Solution Structure of the Human FAAP20 UBZ   PubMed: 25414354

Authors: Wojtaszek, Jessica; Wang, Su; Zhou, Pei

Citation: Wojtaszek, Jessica; Wang, Su; Kim, Hyungjin; Wu, Qinglin; D'Andrea, Alan; Zhou, Pei. "Ubiquitin recognition by FAAP20 expands the complex interface beyond the canonical UBZ domain"  Nucleic Acids Res. 42, 13997-14005 (2014).

Assembly members:
UBZ, polymer, 44 residues, 4853.517 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
UBZ: SHMGAAALRSCPMCQKEFAP RLTQLDVDSHLAQCLAESTE DVTW

Data sets:
Data typeCount
13C chemical shifts142
15N chemical shifts40
1H chemical shifts283

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human FAAP20 UBZ1
2ZINC ION2

Entities:

Entity 1, Human FAAP20 UBZ 44 residues - 4853.517 Da.

Residues 1-3 represent non-native residues leftover after protease cleavage removal of an affinity tag; residues 4-44 represent the UBZ domain of human FAAP20 (140-180).

1   SERHISMETGLYALAALAALALEUARGSER
2   CYSPROMETCYSGLNLYSGLUPHEALAPRO
3   ARGLEUTHRGLNLEUASPVALASPSERHIS
4   LEUALAGLNCYSLEUALAGLUSERTHRGLU
5   ASPVALTHRTRP

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Human FAAP20 UBZ, [U-100% 15N], 1.5 mM; sodium phosphate 25 mM; potassium chloride 100 mM; DTT 10 mM; sodium azide 0.05%

sample_2: Human FAAP20 UBZ, [U-100% 13C; U-100% 15N], 1.5 mM; sodium phosphate 25 mM; potassium chloride 100 mM; DTT 10 mM; sodium azide 0.05%

sample_3: Human FAAP20 UBZ, [U-100% 13C; U-100% 15N], 1.5 mM; sodium phosphate 25 mM; potassium chloride 100 mM; DTT 10 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 0.1125 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D sparse-sampled HNCOsample_2isotropicsample_conditions_1
3D sparse-sampled HNCAsample_2isotropicsample_conditions_1
3D sparse-sampled HNCOCAsample_2isotropicsample_conditions_1
3D sparse-sampled HNCACBsample_2isotropicsample_conditions_1
3D sparse-sampled HNCOCACBsample_2isotropicsample_conditions_1
3D sparse-sampled HACANHsample_2isotropicsample_conditions_1
3D sparse-sampled HACACONHsample_2isotropicsample_conditions_1
4D sparse-sampled HCccoNH TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
4D sparse-sampled HCCH TOCSYsample_3isotropicsample_conditions_1
4D sparse-sampled CHCH NOESYsample_3isotropicsample_conditions_1
4D sparse-sampled CHNH NOESYsample_2isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

XEASY, Bartels et al. - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

SPARKY, Goddard - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SCRUB, Coggins and Zhou - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

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