BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25399

Title: 1H, 15N, 13C resonance assignment of the aortic medial amyloid protein medin.   PubMed: 26377205

Authors: Davies, Hannah; Phelan, Marie; Madine, Jill

Citation: Davies, Hannah; Phelan, Marie; Madine, Jilllian. "1H, 15N and 13C assignment of the amyloidogenic protein medin using fast-pulsing NMR techniques"  Biomol. NMR Assign. 10, 75-77 (2016).

Assembly members:
Medin, polymer, 50 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Medin: RLDKQGNFNAWVAGSYGNDQ WLQVDLGSSKEVTGIITQGA RNFGSVQFVA

Data sets:
Data typeCount
13C chemical shifts144
15N chemical shifts48
1H chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Medin1

Entities:

Entity 1, Medin 50 residues - Formula weight is not available

1   ARGLEUASPLYSGLNGLYASNPHEASNALA
2   TRPVALALAGLYSERTYRGLYASNASPGLN
3   TRPLEUGLNVALASPLEUGLYSERSERLYS
4   GLUVALTHRGLYILEILETHRGLNGLYALA
5   ARGASNPHEGLYSERVALGLNPHEVALALA

Samples:

sample_1: Medin, [U-98% 13C; U-98% 15N], 0.4 mM; sodium phosphate 20 mM; sodium chloride 20 mM; urea 8 M; TSP, [U-2H], 2 uM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromatic TROSYsample_1isotropicsample_conditions_1
3D HNCO BESTsample_1isotropicsample_conditions_1
3D HNCACB BESTsample_1isotropicsample_conditions_1
3D HCACO BESTsample_1isotropicsample_conditions_1
3D HN(CO)CACB BESTsample_1isotropicsample_conditions_1
3D HCCH-TOCSY NUSsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1pl6, Bruker Biospin - collection, processing

Analysis v2.3.1, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP Q08431