BMRB Entry 25433

Title:
NMR structure of the first Zinc Finger domain of RBM10
Deposition date:
2015-01-16
Original release date:
2015-01-26
Authors:
Serrano, Pedro; Wuthrich, Kurt; Geralt, Michael
Citation:

Citation: Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of the first Zinc Finger domain of RBM10"  .

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, 4957.872 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMPKINEDWLCNKCGVQNF KRREKCFKCGVPKSEAEQKL PLG

Data sets:
Data typeCount
1H chemical shifts228
13C chemical shifts140
15N chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2ZINC ION2

Entities:

Entity 1, entity_1 43 residues - 4957.872 Da.

1   GLYHISMETPROLYSILEASNGLUASPTRP
2   LEUCYSASNLYSCYSGLYVALGLNASNPHE
3   LYSARGARGGLULYSCYSPHELYSCYSGLY
4   VALPROLYSSERGLUALAGLUGLNLYSLEU
5   PROLEUGLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: entity, [U-99% 13C; U-98% 15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 298 K; pH: 6.0; pressure: 1 atm; ionic strength: 0.0798 M

Experiments:

NameSampleSample stateSample conditions
APSY 4D-HACANHsample_1isotropicsample_conditions_1
APSY 5D-HACACONHsample_1isotropicsample_conditions_1
APSY 5D-CBCACONHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CNS, Brunger A. T. et.al., Herrman & Wuthrich - refinement, chemical shift assignment, peak picking, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 17386
PDB
DBJ BAA09471 BAA12144 BAC31087 BAC40753 BAC65490
EMBL CAD97933 CAL37951
GB AAH00681 AAH03089 AAH04181 AAH04674 AAH08733
REF NP_001161247 NP_001161248 NP_001191395 NP_001191396 NP_001191397
SP P70501 P98175 Q99KG3
TPG DAA12902 DAA12903
AlphaFold P98175 P70501 Q99KG3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks