BMRB Entry 25470

Title:
Phosphorylation of CB1Cannabinoid Receptor Fourth Intracellular Loop Peptides: Effects on Structure and Function
Deposition date:
2015-02-06
Original release date:
2016-02-15
Authors:
Ganjiwale, Anjali; Eldeeb, Khalil; Chandrashekar, Indu; Cowsik, Sudha; Howlett, Allyn
Citation:

Citation: Ganjiwale, Anjali; Eldeeb, Khalil; Chandrashekar, Indu; Cowsik, Sudha; Howlett, Allyn. "Phosphorylation of CB1Cannabinoid Receptor Fourth Intracellular Loop Peptides: Effects on Structure and Function"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
entity, polymer, 17 residues, 2136.324 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: RXKDLRHAFRSMFPSSE

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 17 residues - 2136.324 Da.

1   ARGSEPLYSASPLEUARGHISALAPHEARG
2   SERMETPHEPROSERSERGLU

Samples:

sample_1: IL4pSER402 peptide 5 mM; SDS, [U-100% 2H], 72 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 5 mM; pH: 4.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert P. - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz