BMRB Entry 25627

Title:
Solution structure of the meiosis-expressed gene 1 (Meig1)
Deposition date:
2015-05-16
Original release date:
2016-01-19
Authors:
Williams, David; Walavalkar, Ninad; Buchwald, William
Citation:

Citation: Li, Wei; Walavalkar, Ninad; Buchwald, William; Teves, Maria; Zhang, Ling; Liu, Junping; Bilinovich, S.; Peterson, Darrell; Strauss, Jerome; Williams, David; Zhang, Zhibing. "Dissecting the structural basis of MEIG1 interaction with PACRG"  Sci. Rep. 6, 18278-18278 (2016).
PubMed: 26726850

Assembly members:

Assembly members:
entity, polymer, 92 residues, 11256.876 Da.

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts410
15N chemical shifts99
1H chemical shifts623

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 92 residues - 11256.876 Da.

1   GLYSERHISMETMETALATHRSERASPVAL
2   LYSPROLYSSERILESERARGALALYSLYS
3   TRPSERGLUGLUILEGLUASNLEUTYRARG
4   PHEGLNGLNALAGLYTYRARGASPGLUILE
5   GLUTYRLYSGLNVALLYSGLNVALALAMET
6   VALASPARGTRPPROGLUTHRGLYTYRVAL
7   LYSLYSLEUGLNARGARGASPASNTHRPHE
8   PHETYRTYRASNLYSGLUARGGLUCYSGLU
9   ASPLYSGLUVALHISLYSVALLYSVALTYR
10   VALTYR

Samples:

sample_1: sodium phosphate 10 mM; Meig1, [U-99% 13C; U-99% 15N], 0.5 – 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 40 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D IPAP-HSQCsample_1anisotropicsample_conditions_1
2D IPAP-HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - data analysis

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks