BMRB Entry 27172

Title:
Ubiquitin V26A Mutant at pH 5.0
Deposition date:
2017-07-11
Original release date:
2017-08-04
Authors:
Bruett, Carter; Soss, Sarah; Flynn, Peter
Citation:

Citation: Bruett, Carter; Soss, Sarah; Flynn, Peter; Bruett, Carter; Soss, Sarah; Flynn, Peter. "Ubiquitin mutant V26A pH 5.0"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
V26A_Ubiquitin, polymer, 82 residues, 9359 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts220
15N chemical shifts73
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubiquitin1

Entities:

Entity 1, Ubiquitin 82 residues - 9359 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNALALYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLYHISHISHISHIS
9   HISHIS

Samples:

sample_1: V26A Ubiquitin, [U-99% 13C; U-99% 15N], .337 mM; D2O, [U-99% 2H], 10 v/v; H2O 90 v/v; sodium azide 0 na; acetic acid 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 5.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

VNMRJ v4.2 Rev A, Varian - collection

Felix v2007, Accelrys Software Inc. - processing

SPARKY v3.115, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Agilent INOVA 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks