BMRB Entry 27196

Title:
Solution-NMR assignment of the Ig-like domain of bacteriophage T5 tail tube protein pb6
Deposition date:
2017-08-02
Original release date:
2017-08-04
Authors:
Paul, Schanda; Kurauskas, Vilius; Breyton, Cecile; Arnaud, Charles-Adrien
Citation:

Citation: Fraga, Hugo; Arnaud, Charles-Adrien; Gauto, Diego; Audin, Maxime; Kurauskas, Vilius; Macek, Pavel; Krichel, Carsten; Guan, Jia-Ying; Boisbouvier, Jerome; Sprangers, Remco; Breyton, Cecile; Schanda, Paul. "Solid-State NMR H-N-(C)-H and H-N-C-C 3D/4D Correlation Experiments for Resonance Assignment of Large Proteins."  Chemphyschem 18, 2697-2703 (2017).
PubMed: 28792111

Assembly members:

Assembly members:
Igdomain, polymer, 103 residues, 10543.7874 Da.

Natural source:

Natural source:   Common Name: Bacteriophage T5   Taxonomy ID: 10726   Superkingdom: Viruses   Kingdom: not available   Genus/species: Bacteriophage T5

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts95
1H chemical shifts95

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Igdomain1

Entities:

Entity 1, Igdomain 103 residues - 10543.7874 Da.

1   ALAVALTHRALAILETHRVALLYSSERALA
2   GLYASNVALTHRTHRLEUASNARGSERALA
3   THRLEUGLNMETSERVALGLUVALTHRPRO
4   SERSERALAARGASNLYSGLUVALTHRTRP
5   ALAILETHRALAGLYASPALAALATHRILE
6   ASNALATHRGLYLEULEUARGALAASPALA
7   SERLYSTHRGLYALAVALTHRVALGLUALA
8   THRALALYSASPGLYSERGLYVALLYSGLY
9   THRLYSVALILETHRVALTHRALAGLYGLY
10   GLUASNLEUTYRPHEGLNGLYHISHISHIS
11   HISHISHIS

Samples:

Igdomain_13C15N: Igdomain, [U-100% 13C; U-100% 15N], 10.9 ± 0.5000 kg/m3; MES buffer 10.0 ± 0.0010 mM; MgCl2 2.0 ± 0.0001 mM; NaCl 100.0 ± 0.0050 mM

sample1: ionic strength: 0.110 M; pH: 6.500; pressure: 1.000 atm; temperature: 301.000 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOIgdomain_13C15Nisotropicsample1
3D HN(CO)CAIgdomain_13C15Nisotropicsample1
Bruker_7_HNCOCACB (H[N[co[ca[CB]]]])Igdomain_13C15Nisotropicsample1
2D 1H-15N HSQC/HMQCIgdomain_13C15Nisotropicsample1
3D HNCAIgdomain_13C15Nisotropicsample1
3D HNCACBIgdomain_13C15Nisotropicsample1

Software:

CcpNmr_Analysis v2.3, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks