BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 27346

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for H2A in mono-nucleosome   PubMed: 29465771

Authors: Xiang, Shengqi; le Paige, Ulric

Citation: Xiang, Shengqi; le Paige, Ulric; Horn, Velten; Houben, Klaartje; Baldus, Marc; van Ingen, Hugo. "Site-Specific Studies of Nucleosome Interactions by Solid-State NMR"  Angew. Chem. Int. Ed. Engl. 57, 4571-4575 (2018).

Assembly members:
H2A-Nucleosome, polymer, 123 residues, Formula weight is not available

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
H2A-Nucleosome: SGRGKGGKVKGKAKSRSNRA GLQFPVGRIHRLLRKGNYAE RVGAGAPVYLAAVMEYLAAE VLELAGNAARDNKKTRIIPR HLQLAIRNDEELNKLLSGVT IAQGGVLPNIQAVLLPKKTE KKA

Data sets:
Data typeCount
13C chemical shifts188
15N chemical shifts89
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1H2A1

Entities:

Entity 1, H2A 123 residues - Formula weight is not available

1   SERGLYARGGLYLYSGLYGLYLYSVALLYS
2   GLYLYSALALYSSERARGSERASNARGALA
3   GLYLEUGLNPHEPROVALGLYARGILEHIS
4   ARGLEULEUARGLYSGLYASNTYRALAGLU
5   ARGVALGLYALAGLYALAPROVALTYRLEU
6   ALAALAVALMETGLUTYRLEUALAALAGLU
7   VALLEUGLULEUALAGLYASNALAALAARG
8   ASPASNLYSLYSTHRARGILEILEPROARG
9   HISLEUGLNLEUALAILEARGASNASPGLU
10   GLULEUASNLYSLEULEUSERGLYVALTHR
11   ILEALAGLNGLYGLYVALLEUPROASNILE
12   GLNALAVALLEULEUPROLYSLYSTHRGLU
13   LYSLYSALA

Samples:

sample_1: H2A-Nucleosome, [U-100% 13C; U-100% 15N; U-80% 2H], 2 mg; H2A-Nucleosome, [U-100% 13C; U-100% 15N; U-80% 2H], 250 ug; H2B-Nucleosome 250 ug; H3-Nucleosome 250 ug; H4-Nucleosome 250 ug; 601DNA-Nucleosome 1000 ug

sample_conditions_1: ionic strength: 30 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1
3D CANHsample_1anisotropicsample_conditions_1
3D CA(CO)NHsample_1anisotropicsample_conditions_1
3D CONHsample_1anisotropicsample_conditions_1
3D CO(CA)NHsample_1anisotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection

NMRPipe v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.13, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

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