BMRB Entry 27434

Title:
1H chemical shift assignment of the Interfacial contact of phycocyanobilin chromophore in the plant phytochrome A in its Pfr state.
Deposition date:
2018-03-26
Original release date:
2018-05-22
Authors:
Song, Chen
Citation:

Citation: Song, Chen; Mroginski, Maria Andrea; Lang, Christina; Kopycki, Jakub; Gartner, Wolfgang; Matysik, Jorg; Hughes, Jon. "3D Structures of Plant Phytochrome A as Pr and Pfr From Solid-State NMR: Implications for Molecular Function"  Front. Plant Sci. 9, 498-498 (2018).
PubMed: 29740459

Assembly members:

Assembly members:
oat_phyA3, polymer, 527 residues, Formula weight is not available
entity_PCB, non-polymer, 448.169 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: n.a.

Data sets:
Data typeCount
1H chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1oat phyA31
2PCB2

Entities:

Entity 1, oat phyA3 527 residues - Formula weight is not available

1   SERGLULYSVALILEALATYRLEUGLNHSE
2   ILEGLNLYSGLYLYSLEUILEGLNTHRPHE
3   GLYCYSLEULEUALALEUASPGLULYSSER
4   PHEASNVALILEALAPHESERGLUASNALA
5   PROGLUMETLEUTHRTHRVALSERHSDALA
6   VALPROSERVALASPASPPROPROARGLEU
7   GLYILEGLYTHRASNVALARGSERLEUPHE
8   SERASPGLNGLYALATHRALALEUHSDLYS
9   ALALEUGLYPHEALAASPVALSERLEULEU
10   ASNPROILELEUVALGLNCYSLYSTHRSER
11   GLYLYSPROPHETYRALAILEVALHSDARG
12   ALATHRGLYCYSLEUVALVALASPPHEGLU
13   PROVALLYSPROTHRGLUPHEPROALATHR
14   ALAALAGLYALALEUGLNSERTYRLYSLEU
15   ALAALALYSALAILESERLYSILEGLNSER
16   LEUPROGLYGLYSERMETGLUVALLEUCYS
17   ASNTHRVALVALLYSGLUVALPHEASPLEU
18   THRGLYTYRASPARGVALMETALATYRLYS
19   PHEHSDGLUASPASPHSDGLYGLUVALPHE
20   SERGLUILETHRLYSPROGLYLEUGLUPRO
21   TYRLEUGLYLEUHSDTYRPROALATHRASP
22   ILEPROGLNALAALAARGLEULEUPHEMET
23   LYSASNLYSVALARGMETILECYSASPCYS
24   ARGALAARGSERILELYSVALILEGLUALA
25   GLUALALEUPROPHEASPILESERLEUCYS
26   GLYSERALALEUARGALAPROHSESERCYS
27   HSELEUGLNTYRMETGLUASNMETASNSER
28   ILEALASERLEUVALMETALAVALVALVAL
29   ASNGLUASNGLUGLUASPASPGLUALAGLU
30   SERGLUGLNPROALAGLNGLNGLNLYSLYS
31   LYSLYSLEUTRPGLYLEULEUVALCYSHSE
32   HSDGLUSERPROARGTYRVALPROPHEPRO
33   LEUARGTYRALACYSGLUPHELEUALAGLN
34   VALPHEALAVALHSDVALASNARGGLUPHE
35   GLULEUGLULYSGLNLEUARGGLULYSASN
36   ILELEULYSMETGLNTHRMETLEUSERASP
37   METLEUPHEARGGLUALASERPROLEUTHR
38   ILEVALSERGLYTHRPROASNILEMETASP
39   LEUVALLYSCYSASPGLYALAALALEULEU
40   TYRGLYGLYLYSVALTRPARGLEUARGASN
41   ALAPROTHRGLUSERGLNILEHSDASPILE
42   ALAPHETRPLEUSERASPVALHSDARGASP
43   SERTHRGLYLEUSERTHRASPSERLEUHSD
44   ASPALAGLYTYRPROGLYALAALAALALEU
45   GLYASPMETILECYSGLYMETALAVALALA
46   LYSILEASNSERLYSASPILELEUPHETRP
47   PHEARGSERHSDTHRALAALAGLUILEARG
48   TRPGLYGLYALALYSASNASPPROSERASP
49   METASPASPSERARGARGMETHSDPROARG
50   LEUSERPHELYSALAPHELEUGLUVALVAL
51   LYSMETLYSSERLEUPROTRPSERASPTYR
52   GLUMETASPALAILEHSDSERLEUGLNLEU
53   ILELEUARGGLYTHRLEUASN

Entity 2, PCB - C14 H10 Cl4 O4 S2 - 448.169 Da.

1   PCB

Samples:

sample_1: phycocyanobilin (PCB), [U-100% 13C; U-100% 15N], 2 mM

sample_conditions_1: temperature: 233 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker AMX 750 MHz