BMRB Entry 27492

Title:
1H Chemical Shift Assignments for SG5 variant of Schistocerca gregaria protease inhibitor 2
Deposition date:
2018-05-23
Original release date:
2019-02-07
Authors:
Sebak, Fanni; Bodor, Andrea
Citation:

Citation: Boros, Eszter; Sebak, Fanni; Heja, David; Szakacs, David; Zboray, Katalin; Schlosser, Gitta; Micsonai, Andras; Kardos, Jozsef; Bodor, Andrea; Pal, Gabor. "Directed Evolution of Canonical Loops and Their Swapping between Unrelated Serine Proteinase Inhibitors Disprove the Interscaffolding Additivity Model"  J. Mol. Biol. 431, 557-575 (2019).
PubMed: 30543823

Assembly members:

Assembly members:
SGPI-2_variant, polymer, 38 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: desert locust   Taxonomy ID: 7010   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Schistocerca gregaria

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: M13KO7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SGPI-2_variant: GSGEVTCEPGTTFKDKCNTC RCGSDGKSAGCTFILCPQ

Data sets:
Data typeCount
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SGPI-2 variant1

Entities:

Entity 1, SGPI-2 variant 38 residues - Formula weight is not available

'Residues -2 - 0 represent a cloning artifact.

1   GLYSERGLYGLUVALTHRCYSGLUPROGLY
2   THRTHRPHELYSASPLYSCYSASNTHRCYS
3   ARGCYSGLYSERASPGLYLYSSERALAGLY
4   CYSTHRPHEILELEUCYSPROGLN

Samples:

sample_1: SGPI-2 variant 1 mM; MES 10 mM; sodium chloride 50 mM; DSS 5 uL

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz