BMRB Entry 27562

Title:
GB1 crystalline protein U-13C,15N
Deposition date:
2018-07-26
Original release date:
2018-11-15
Authors:
Pawlak, Tomasz
Citation:

Citation: Paluch, Piotr; Pawlak, Tomasz; awniczak, Karol; Trebosc, Julien; Lafon, Olivier; Amoreux, Jean; Potrzebowski, Marek. "Simple and Robust Study of Backbone Dynamics of Crystalline Proteins Employing 1H-15N Dipolar Coupling Dispersion"  J. Phys. Chem. B. 122, 8146-8156 (2018).
PubMed: 30070484

Assembly members:

Assembly members:
GB1, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: na   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts149
15N chemical shifts51
1H chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GB1, 11
2GB1, 21
3GB1, 31
4GB1, 41

Entities:

Entity 1, GB1, 1 56 residues - Formula weight is not available

1   METGLNTYRLYSLEUILELEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRPHE

Samples:

sample_1: GB1, [U-13C; U-15N; U-2H], NH back exchage, mM

sample_conditions_1: pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D hNHsample_1solidsample_conditions_1
2D hNCOsample_1solidsample_conditions_1
2D hNCAsample_1solidsample_conditions_1
3D hCONHsample_1solidsample_conditions_1
3D hCANHsample_1solidsample_conditions_1
3D hCOcaNHsample_1solidsample_conditions_1
3D hcoCAcoNHsample_1solidsample_conditions_1
3D hcaCBcaNHsample_1solissample_conditions_1
3D hcaCBcacoNHsample_1solissample_conditions_1

Software:

SPARKY v3, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks