BMRB Entry 27600

Title:
Ghrelin Binding at its G Protein-Coupled Receptor
Deposition date:
2018-09-07
Original release date:
2019-01-29
Authors:
Bender, Brian; Vortmeier, Gerrit; Ernicke, Stefan; Bosse, Mathias; Kaiser, Anette; Els-Heindl, Sylvia; Krug, Ulrike; Beck-Sickinger, Annette; Meiler, Jens; Huster, Daniel
Citation:

Citation: Bender, Brian; Vortmeier, Gerrit; Ernicke, Stefan; Bosse, Mathias; Kaiser, Anette; Els-Heindl, Sylvia; Krug, Ulrike; Beck-Sickinger, Annette; Meiler, Jens; Huster, Daniel. "Structural Model of Ghrelin Bound to its G Protein-Coupled Receptor"  Structure 27, 537-544 (2019).
PubMed: 30686667

Assembly members:

Assembly members:
Ghrelin, polymer, 28 residues, Formula weight is not available
GHSR, polymer, 380 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Data sets:
Data typeCount
13C chemical shifts32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ghrelin1
2GHSR2

Entities:

Entity 1, Ghrelin 28 residues - Formula weight is not available

1   GLYSERSERPHELEUSERPROGLUHISGLN
2   ARGVALGLNGLNARGLYSGLUSERLYSLYS
3   PROPROALALYSLEUGLNPROARG

Entity 2, GHSR 380 residues - Formula weight is not available

1   GLYHISSERARGMETTRPASNALATHRPRO
2   SERGLUGLUPROGLYPHEASNLEUTHRLEU
3   ALAASPLEUASPTRPASPALASERPROGLY
4   ASNASPSERLEUGLYASPGLULEULEUGLN
5   LEUPHEPROALAPROLEULEUALAGLYVAL
6   THRALATHRCYSVALALALEUPHEVALVAL
7   GLYILEALAGLYASNLEULEUTHRMETLEU
8   VALVALSERARGPHEARGGLULEUARGTHR
9   THRTHRASNLEUTYRLEUSERSERMETALA
10   PHESERASPLEULEUILEPHELEUCYSMET
11   PROLEUASPLEUVALARGLEUTRPGLNTYR
12   ARGPROTRPASNPHEGLYASPLEULEUCYS
13   LYSLEUPHEGLNPHEVALSERGLUSERCYS
14   THRTYRALATHRVALLEUTHRILETHRALA
15   LEUSERVALGLUARGTYRPHEALAILECYS
16   PHEPROLEUARGALALYSVALVALVALTHR
17   LYSGLYARGVALLYSLEUVALILEPHEVAL
18   ILETRPALAVALALAPHECYSSERALAGLY
19   PROILEPHEVALLEUVALGLYVALGLUHIS
20   GLUASNGLYTHRASPPROTRPASPTHRASN
21   GLUCYSARGPROTHRGLUPHEALAVALARG
22   SERGLYLEULEUTHRVALMETVALTRPVAL
23   SERSERILEPHEPHEPHELEUPROVALPHE
24   CYSLEUTHRVALLEUTYRSERLEUILEGLY
25   ARGLYSLEUTRPARGARGARGARGGLYASP
26   ALAVALVALGLYALASERLEUARGASPGLN
27   ASNHISLYSGLNTHRVALLYSMETLEUALA
28   VALVALVALPHEALAPHEILELEUCYSTRP
29   LEUPROPHEHISVALGLYARGTYRLEUPHE
30   SERLYSSERPHEGLUPROGLYSERLEUGLU
31   ILEALAGLNILESERGLNTYRCYSASNLEU
32   VALSERPHEVALLEUPHETYRLEUSERALA
33   ALAILEASNPROILELEUTYRASNILEMET
34   SERLYSLYSTYRARGVALALAVALPHEARG
35   LEULEUGLYPHEGLUPROPHESERGLNARG
36   LYSLEUSERTHRLEULYSASPGLUSERSER
37   ARGALATRPTHRGLUSERSERILEASNTHR
38   LEUGLUHISHISHISHISHISHISHISHIS

Samples:

sample_1: Ghrelin, G1,S2,S3,F4,L5,S6,P7,E8,Q10,V12,Q14,P22,A23,P27 [U-13C; U-15N], 0.2 uM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D SQ/DQ (Inadequate type)sample_1solidsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz