BMRB Entry 27611

Title:
Novel antimicrobial peptides from the Arctic polychaeta Nicomache minor share structural similarities with amphibian host-defense peptides
Deposition date:
2018-09-14
Original release date:
2019-01-09
Authors:
Panteleev, Pavel; Tsarev, Andrey; Bolosov, Ilia; Paramonov, Alexander; Marggraf, Mariana; Sychev, Sergey; Shenkarev, Zakhar; Ovchinnikova, Tatiana
Citation:

Citation: Panteleev, Pavel; Tsarev, Andrey; Bolosov, Ilia; Paramonov, Alexander; Marggraf, Mariana; Sychev, Sergey; Shenkarev, Zakhar; Ovchinnikova, Tatiana. "Novel Antimicrobial Peptides from the Arctic Polychaeta"  Mar. Drugs 16, E401-E401 (2018).
PubMed: 30360541

Assembly members:

Assembly members:
Nicomacin, polymer, 33 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEM-T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nicomacin: GFWSSVWDGAKNVGTAIIKN AKVCVYAVCVSHK

Data sets:
Data typeCount
13C chemical shifts79
1H chemical shifts209

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nicomacin1

Entities:

Entity 1, Nicomacin 33 residues - Formula weight is not available

1   GLYPHETRPSERSERVALTRPASPGLYALA
2   LYSASNVALGLYTHRALAILEILELYSASN
3   ALALYSVALCYSVALTYRALAVALCYSVAL
4   SERHISLYS

Samples:

sample_1: Nicomacin 0.4 mM

sample_conditions_1: ionic strength: 20 mM; pH: 4.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz